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Induction of telomere-mediated chromosomal truncation and stability of truncated chromosomes in Arabidopsis thaliana.

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Title: Induction of telomere-mediated chromosomal truncation and stability of truncated chromosomes in Arabidopsis thaliana.
Authors: Teo, Chee How1, Ma, Lu1, Kapusi, Eszter1, Hensel, Götz1, Kumlehn, Jochen1, Schubert, Ingo1, Houben, Andreas1, Mette, Michael Florian
Source: Plant Journal. Oct2011, Vol. 68 Issue 1, p28-39. 12p.
Subjects: Arabidopsis thaliana, Plant chromosomes, Telomeres, Centromere, Plant breeding, Polymerase chain reaction, Fluorescence in situ hybridization
Abstract: Summary Minichromosomes possess functional centromeres and telomeres and thus should be stably inherited. They offer an enormous opportunity to plant biotechnology as they have the potential to simultaneously transfer and stably express multiple genes. Segregating independently of host chromosomes, they provide a platform for accelerating plant breeding. Following a top-down approach, we truncated endogenous chromosomes in Arabidopsis thaliana by Agrobacterium-mediated transfer of T-DNA constructs containing telomere sequences. Blocks of A. thaliana telomeric repeats were inserted into a binary vector suitable for stable transformation. After transfer of these constructs into the natural tetraploid A. thaliana accession Wa-1, chromosome truncation by T-DNA-induced de novo formation of telomeres could be confirmed by DNA gel blot analysis, PCR (polymerase chain reaction), and fluorescence in situ hybridisation. The addition of new telomere repeats in this process could start alternatively from within the T-DNA-derived telomere repeats or from adjacent sequences close to the right border of the T-DNA. Truncated chromosomes were transmissible in sexual reproduction, but were inherited at rates lower than expected according to Mendelian rules. [ABSTRACT FROM AUTHOR]
Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 65971742
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Induction of telomere-mediated chromosomal truncation and stability of truncated chromosomes in Arabidopsis thaliana.
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  Data: <searchLink fieldCode="AR" term="%22Teo%2C+Chee+How%22">Teo, Chee How</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ma%2C+Lu%22">Ma, Lu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kapusi%2C+Eszter%22">Kapusi, Eszter</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hensel%2C+Götz%22">Hensel, Götz</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kumlehn%2C+Jochen%22">Kumlehn, Jochen</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Schubert%2C+Ingo%22">Schubert, Ingo</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Houben%2C+Andreas%22">Houben, Andreas</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mette%2C+Michael+Florian%22">Mette, Michael Florian</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. Oct2011, Vol. 68 Issue 1, p28-39. 12p.
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  Data: <searchLink fieldCode="DE" term="%22Arabidopsis+thaliana%22">Arabidopsis thaliana</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+chromosomes%22">Plant chromosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Telomeres%22">Telomeres</searchLink><br /><searchLink fieldCode="DE" term="%22Centromere%22">Centromere</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+breeding%22">Plant breeding</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+in+situ+hybridization%22">Fluorescence in situ hybridization</searchLink>
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  Label: Abstract
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  Data: Summary Minichromosomes possess functional centromeres and telomeres and thus should be stably inherited. They offer an enormous opportunity to plant biotechnology as they have the potential to simultaneously transfer and stably express multiple genes. Segregating independently of host chromosomes, they provide a platform for accelerating plant breeding. Following a top-down approach, we truncated endogenous chromosomes in Arabidopsis thaliana by Agrobacterium-mediated transfer of T-DNA constructs containing telomere sequences. Blocks of A. thaliana telomeric repeats were inserted into a binary vector suitable for stable transformation. After transfer of these constructs into the natural tetraploid A. thaliana accession Wa-1, chromosome truncation by T-DNA-induced de novo formation of telomeres could be confirmed by DNA gel blot analysis, PCR (polymerase chain reaction), and fluorescence in situ hybridisation. The addition of new telomere repeats in this process could start alternatively from within the T-DNA-derived telomere repeats or from adjacent sequences close to the right border of the T-DNA. Truncated chromosomes were transmissible in sexual reproduction, but were inherited at rates lower than expected according to Mendelian rules. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1111/j.1365-313X.2011.04662.x
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      – Code: eng
        Text: English
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        PageCount: 12
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      – SubjectFull: Arabidopsis thaliana
        Type: general
      – SubjectFull: Plant chromosomes
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      – SubjectFull: Telomeres
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      – SubjectFull: Centromere
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      – SubjectFull: Plant breeding
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      – SubjectFull: Polymerase chain reaction
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      – SubjectFull: Fluorescence in situ hybridization
        Type: general
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      – TitleFull: Induction of telomere-mediated chromosomal truncation and stability of truncated chromosomes in Arabidopsis thaliana.
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              Text: Oct2011
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