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Potassium-doped carbon nanotubes toward the direct electrochemistry of cholesterol oxidase and its application in highly sensitive cholesterol biosensor

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Title: Potassium-doped carbon nanotubes toward the direct electrochemistry of cholesterol oxidase and its application in highly sensitive cholesterol biosensor
Authors: Li, Xiao-Rong1, Xu, Jing-Juan xujj@nju.edu.cn, Chen, Hong-Yuan1
Source: Electrochimica Acta. Oct2011, Vol. 56 Issue 25, p9378-9385. 8p.
Subjects: Potassium, Semiconductor doping, Carbon nanotubes, Electrochemical sensors, Oxidases, Cholesterol, Oxidation-reduction reaction, Spectrophotometry
Abstract: Abstract: We demonstrate herein a newly developed serum total cholesterol biosensor by using the direct electron transfer of cholesterol oxidase (ChOx), which is based on the immobilization of cholesterol oxidase and cholesterol esterase (ChEt) on potassium-doped multi-walled carbon nanotubes (KMWNTs) modified electrodes. The KMWNTs accelerate the electron transfer from electrode surface to the immobilized ChOx, achieving the direct electrochemistry of ChOx and maintaining its bioactivity. As a new platform in cholesterol analysis, the resulting electrode (ChOx/KMWNTs/GCE) exhibits a sensitive response to free cholesterol, with a linear range of 0.050–16.0μmolL−1 and a detection limit of 5.0nmolL−1 (S/N=3). Coimmobilization of ChEt and ChOx (ChEt/ChOx/KMWNTs/GCE) allows the determination of both free cholesterol and esterified cholesterol. The resulting biosensor shows the same linear range of 0.050–16.0μmolL−1 for free cholesterol and cholesteryl oleate, with the detection limit of 10.0 and 12.0nmolL−1 (S/N=3), respectively. The concentrations of total (free and esterified) cholesterol in human serum samples, determined by using the techniques developed in the present study, are in good agreement with those determined by the well-established techniques using the spectrophotometry. [Copyright &y& Elsevier]
Copyright of Electrochimica Acta is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 66160432
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PubTypeId: academicJournal
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  Data: Potassium-doped carbon nanotubes toward the direct electrochemistry of cholesterol oxidase and its application in highly sensitive cholesterol biosensor
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Xiao-Rong%22">Li, Xiao-Rong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xu%2C+Jing-Juan%22">Xu, Jing-Juan</searchLink><i> xujj@nju.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Hong-Yuan%22">Chen, Hong-Yuan</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Electrochimica+Acta%22">Electrochimica Acta</searchLink>. Oct2011, Vol. 56 Issue 25, p9378-9385. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Potassium%22">Potassium</searchLink><br /><searchLink fieldCode="DE" term="%22Semiconductor+doping%22">Semiconductor doping</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+nanotubes%22">Carbon nanotubes</searchLink><br /><searchLink fieldCode="DE" term="%22Electrochemical+sensors%22">Electrochemical sensors</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidases%22">Oxidases</searchLink><br /><searchLink fieldCode="DE" term="%22Cholesterol%22">Cholesterol</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidation-reduction+reaction%22">Oxidation-reduction reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Spectrophotometry%22">Spectrophotometry</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Abstract: We demonstrate herein a newly developed serum total cholesterol biosensor by using the direct electron transfer of cholesterol oxidase (ChOx), which is based on the immobilization of cholesterol oxidase and cholesterol esterase (ChEt) on potassium-doped multi-walled carbon nanotubes (KMWNTs) modified electrodes. The KMWNTs accelerate the electron transfer from electrode surface to the immobilized ChOx, achieving the direct electrochemistry of ChOx and maintaining its bioactivity. As a new platform in cholesterol analysis, the resulting electrode (ChOx/KMWNTs/GCE) exhibits a sensitive response to free cholesterol, with a linear range of 0.050–16.0μmolL−1 and a detection limit of 5.0nmolL−1 (S/N=3). Coimmobilization of ChEt and ChOx (ChEt/ChOx/KMWNTs/GCE) allows the determination of both free cholesterol and esterified cholesterol. The resulting biosensor shows the same linear range of 0.050–16.0μmolL−1 for free cholesterol and cholesteryl oleate, with the detection limit of 10.0 and 12.0nmolL−1 (S/N=3), respectively. The concentrations of total (free and esterified) cholesterol in human serum samples, determined by using the techniques developed in the present study, are in good agreement with those determined by the well-established techniques using the spectrophotometry. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Electrochimica Acta is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.electacta.2011.08.020
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      – Code: eng
        Text: English
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        PageCount: 8
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        Type: general
      – SubjectFull: Semiconductor doping
        Type: general
      – SubjectFull: Carbon nanotubes
        Type: general
      – SubjectFull: Electrochemical sensors
        Type: general
      – SubjectFull: Oxidases
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      – SubjectFull: Cholesterol
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      – SubjectFull: Oxidation-reduction reaction
        Type: general
      – SubjectFull: Spectrophotometry
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      – TitleFull: Potassium-doped carbon nanotubes toward the direct electrochemistry of cholesterol oxidase and its application in highly sensitive cholesterol biosensor
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            NameFull: Li, Xiao-Rong
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              Text: Oct2011
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