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A Novel E2F-Iike Protein Involved in Transcriptional Activation of Cyst Wall Protein Genes in Giardia Iamblia.

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Title: A Novel E2F-Iike Protein Involved in Transcriptional Activation of Cyst Wall Protein Genes in Giardia Iamblia.
Authors: Li-Hsin Su1, Yu-Jiao Pan1, Yu-Chang Huang1, Chao-Cheng Cho1, Chia-Wei Chen1, Shao-Wei Huang1, Sheng-Fung Chuang1, Chin-Hung Sun1 chinhsun@ntu.edu.tw
Source: Journal of Biological Chemistry. 9/30/2011, Vol. 286 Issue 39, p34101-34120. 20p.
Subjects: Genetic transcription, Giardia lamblia, Gene expression, Messenger RNA, Cysts (Pathology), Cell proliferation
Abstract: Giardia lamblia differentiates into resistant walled cysts for survival outside the host and transmission. During encystation, synthesis of cyst wall proteins is coordinately induced. The E2F family of transcription factors in higher eukaryotes is involved in cell cycle progression and cell differentiation. We asked whether Giardia has E2F-like genes and whether they influence gene expression during Giardia encystation. Blast searches of the Giardia genome database identified one gene (e2fl) encod- ing a putatiLve E2F protein with two putative DNA-binding domains. We found that the e2fl gene expression levels in- creased significantly during encystation. Epitope-tagged E2F1 was found to localize to nuclei. Recombinant E2F1 specifically bound to the thyrnidine kinase and cwpl -3 gene promoters. E2F1 contains several key residues for DNA binding, and muta- tion analysis revealed that its binding sequence is similar to those of the known E2F family proteins. The E2F1-binding sequences were positive cis-acting elements of the thymidine kinase and cwpl promoters. We also found that E2F1 transacti- vated the thyrnidine kinase and cwpl promoters through its binding sequences in vivo. Interestingly, E2F1 overexpression resulted in a significant increase of the levels of CWP1 protein, cwpl-3 gene mRNA, and cyst formation. We also found E2F1 can interact with Myb2, a transcription factor that coordinate up-regulates the cwpl-3 genes during encystation. Our results suggest that: E2F family has been conserved during evolution and that E211 is an important transcription factor in regulation of the Giardia cwp genes, which are key to Giardia differentia- tion into cysts. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A Novel E2F-Iike Protein Involved in Transcriptional Activation of Cyst Wall Protein Genes in Giardia Iamblia.
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  Data: <searchLink fieldCode="AR" term="%22Li-Hsin+Su%22">Li-Hsin Su</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yu-Jiao+Pan%22">Yu-Jiao Pan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yu-Chang+Huang%22">Yu-Chang Huang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chao-Cheng+Cho%22">Chao-Cheng Cho</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chia-Wei+Chen%22">Chia-Wei Chen</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shao-Wei+Huang%22">Shao-Wei Huang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sheng-Fung+Chuang%22">Sheng-Fung Chuang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chin-Hung+Sun%22">Chin-Hung Sun</searchLink><relatesTo>1</relatesTo><i> chinhsun@ntu.edu.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 9/30/2011, Vol. 286 Issue 39, p34101-34120. 20p.
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  Data: <searchLink fieldCode="DE" term="%22Genetic+transcription%22">Genetic transcription</searchLink><br /><searchLink fieldCode="DE" term="%22Giardia+lamblia%22">Giardia lamblia</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger+RNA%22">Messenger RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Cysts+%28Pathology%29%22">Cysts (Pathology)</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Giardia lamblia differentiates into resistant walled cysts for survival outside the host and transmission. During encystation, synthesis of cyst wall proteins is coordinately induced. The E2F family of transcription factors in higher eukaryotes is involved in cell cycle progression and cell differentiation. We asked whether Giardia has E2F-like genes and whether they influence gene expression during Giardia encystation. Blast searches of the Giardia genome database identified one gene (e2fl) encod- ing a putatiLve E2F protein with two putative DNA-binding domains. We found that the e2fl gene expression levels in- creased significantly during encystation. Epitope-tagged E2F1 was found to localize to nuclei. Recombinant E2F1 specifically bound to the thyrnidine kinase and cwpl -3 gene promoters. E2F1 contains several key residues for DNA binding, and muta- tion analysis revealed that its binding sequence is similar to those of the known E2F family proteins. The E2F1-binding sequences were positive cis-acting elements of the thymidine kinase and cwpl promoters. We also found that E2F1 transacti- vated the thyrnidine kinase and cwpl promoters through its binding sequences in vivo. Interestingly, E2F1 overexpression resulted in a significant increase of the levels of CWP1 protein, cwpl-3 gene mRNA, and cyst formation. We also found E2F1 can interact with Myb2, a transcription factor that coordinate up-regulates the cwpl-3 genes during encystation. Our results suggest that: E2F family has been conserved during evolution and that E211 is an important transcription factor in regulation of the Giardia cwp genes, which are key to Giardia differentia- tion into cysts. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1074/jbc.M111.280206
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        Text: English
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        PageCount: 20
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      – SubjectFull: Genetic transcription
        Type: general
      – SubjectFull: Giardia lamblia
        Type: general
      – SubjectFull: Gene expression
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      – SubjectFull: Messenger RNA
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      – SubjectFull: Cysts (Pathology)
        Type: general
      – SubjectFull: Cell proliferation
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      – TitleFull: A Novel E2F-Iike Protein Involved in Transcriptional Activation of Cyst Wall Protein Genes in Giardia Iamblia.
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              Text: 9/30/2011
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