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Electrochemical investigations of fungal cytochrome P450

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Title: Electrochemical investigations of fungal cytochrome P450
Authors: Vatsyayan, Preety1, Chakraborty, Mitun1, Bordoloi, Sandip2, Goswami, Pranab1 pgoswami@iitg.ernet.in
Source: Journal of Electroanalytical Chemistry. Nov2011, Vol. 662 Issue 2, p312-316. 5p.
Subjects: Electrochemistry, Cytochrome P-450, Carbon nanotubes, Aziridines, Silver compounds, Electrocatalysis, Aspergillus, Atomic force microscopy, Oxidation-reduction reaction
Abstract: Abstract: The electrochemical investigations of fungal (Aspergillus terreus MTCC 6324) cytochrome P450 monooxygenase (CYP) (EC 1.14.14.1) immobilized on multi-walled carbon nanotubes–Nafion®–polyethyleneimine (MWCNT-NF/PEI) modified glassy carbon electrode (GCE) were carried out to understand the electrochemistry of the CYP and its application potential in electrochemical-based devices. In deoxygenated condition, the formal potential for Fe(III)/Fe(II) redox couple of CYP was about −0.53V (vs. Ag/AgCl reference electrode, pH 8). A positive shift in the redox potential of the bioelectrode to −0.475V was observed in the presence of oxygen and substrate. The electrocatalytic activity towards n-hexadecane and inhibition with piperonyl butoxide (PBO) confirmed the involvement of Fe(III/II) system of CYP in the redox process. The current response increased linearly from 1μM to 100μM of n-hexadecane with detection limit 0.1μM. The IC50 for PBO was 2.7μM. The surface coverage of CYP immobilized on MWCNT-NF/PEI modified GCE was approximately 3.45×10−10 molcm−2. The electron transfer rate constant (ks ) was 1.0±0.2s−1, indicating facilitation of the electron transfer between CYP and GCE. [Copyright &y& Elsevier]
Copyright of Journal of Electroanalytical Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 67143643
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  Data: Electrochemical investigations of fungal cytochrome P450
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  Data: <searchLink fieldCode="AR" term="%22Vatsyayan%2C+Preety%22">Vatsyayan, Preety</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chakraborty%2C+Mitun%22">Chakraborty, Mitun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bordoloi%2C+Sandip%22">Bordoloi, Sandip</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Goswami%2C+Pranab%22">Goswami, Pranab</searchLink><relatesTo>1</relatesTo><i> pgoswami@iitg.ernet.in</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Electroanalytical+Chemistry%22">Journal of Electroanalytical Chemistry</searchLink>. Nov2011, Vol. 662 Issue 2, p312-316. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Electrochemistry%22">Electrochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Cytochrome+P-450%22">Cytochrome P-450</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+nanotubes%22">Carbon nanotubes</searchLink><br /><searchLink fieldCode="DE" term="%22Aziridines%22">Aziridines</searchLink><br /><searchLink fieldCode="DE" term="%22Silver+compounds%22">Silver compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Electrocatalysis%22">Electrocatalysis</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus%22">Aspergillus</searchLink><br /><searchLink fieldCode="DE" term="%22Atomic+force+microscopy%22">Atomic force microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidation-reduction+reaction%22">Oxidation-reduction reaction</searchLink>
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  Data: Abstract: The electrochemical investigations of fungal (Aspergillus terreus MTCC 6324) cytochrome P450 monooxygenase (CYP) (EC 1.14.14.1) immobilized on multi-walled carbon nanotubes–Nafion®–polyethyleneimine (MWCNT-NF/PEI) modified glassy carbon electrode (GCE) were carried out to understand the electrochemistry of the CYP and its application potential in electrochemical-based devices. In deoxygenated condition, the formal potential for Fe(III)/Fe(II) redox couple of CYP was about −0.53V (vs. Ag/AgCl reference electrode, pH 8). A positive shift in the redox potential of the bioelectrode to −0.475V was observed in the presence of oxygen and substrate. The electrocatalytic activity towards n-hexadecane and inhibition with piperonyl butoxide (PBO) confirmed the involvement of Fe(III/II) system of CYP in the redox process. The current response increased linearly from 1μM to 100μM of n-hexadecane with detection limit 0.1μM. The IC50 for PBO was 2.7μM. The surface coverage of CYP immobilized on MWCNT-NF/PEI modified GCE was approximately 3.45×10−10 molcm−2. The electron transfer rate constant (ks ) was 1.0±0.2s−1, indicating facilitation of the electron transfer between CYP and GCE. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Journal of Electroanalytical Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
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      – Type: doi
        Value: 10.1016/j.jelechem.2011.08.020
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 5
        StartPage: 312
    Subjects:
      – SubjectFull: Electrochemistry
        Type: general
      – SubjectFull: Cytochrome P-450
        Type: general
      – SubjectFull: Carbon nanotubes
        Type: general
      – SubjectFull: Aziridines
        Type: general
      – SubjectFull: Silver compounds
        Type: general
      – SubjectFull: Electrocatalysis
        Type: general
      – SubjectFull: Aspergillus
        Type: general
      – SubjectFull: Atomic force microscopy
        Type: general
      – SubjectFull: Oxidation-reduction reaction
        Type: general
    Titles:
      – TitleFull: Electrochemical investigations of fungal cytochrome P450
        Type: main
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            NameFull: Vatsyayan, Preety
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            NameFull: Chakraborty, Mitun
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            NameFull: Bordoloi, Sandip
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            NameFull: Goswami, Pranab
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              Text: Nov2011
              Type: published
              Y: 2011
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            – TitleFull: Journal of Electroanalytical Chemistry
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