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Comparison of blood serum peptide enrichment methods by Tricine SDS-PAGE and mass spectrometry

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Title: Comparison of blood serum peptide enrichment methods by Tricine SDS-PAGE and mass spectrometry
Authors: Biosa, Grazia, Addis, Maria Filippa, Tanca, Alessandro, Pisanu, Salvatore, Roggio, Tonina, Uzzau, Sergio, Pagnozzi, Daniela pagnozzi@portocontericerche.it
Source: Journal of Proteomics. Dec2011, Vol. 75 Issue 1, p93-99. 7p.
Subjects: Serum, Peptides, Pathological physiology, Ultrafiltration, Biomarkers, Polyacrylamide gel electrophoresis, Mass spectrometry
Abstract: Abstract: Characterisation of blood serum peptides can provide valuable information on physiological and pathological processes. However, the analysis of raw serum samples by MS results in the identification of a limited number of peptides. In order to improve sensitivity, many peptide enrichment methods have been proposed during the last ten years. Here, we present a comparison of fractionation methods aimed to simplify analysis of small proteins and peptides in blood serum, one of the most promising sources of putative biomarkers. Specifically, three methods based on ultrafiltration, differential precipitation, and peptide ligand libraries (ProteoMiner) were evaluated for the enrichment of peptides and low molecular weight proteins, as demonstrated by Tricine SDS-PAGE and subsequent LC-MS/MS (GeLC-MS/MS). As a result, differential solubilisation (DS) allowed the identification of the highest number of peptides. Moreover, the DS method enabled also the quantitative comparison of samples, producing fundamental information in biomarker discovery approaches. [Copyright &y& Elsevier]
Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 67627124
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Comparison of blood serum peptide enrichment methods by Tricine SDS-PAGE and mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Biosa%2C+Grazia%22">Biosa, Grazia</searchLink><br /><searchLink fieldCode="AR" term="%22Addis%2C+Maria+Filippa%22">Addis, Maria Filippa</searchLink><br /><searchLink fieldCode="AR" term="%22Tanca%2C+Alessandro%22">Tanca, Alessandro</searchLink><br /><searchLink fieldCode="AR" term="%22Pisanu%2C+Salvatore%22">Pisanu, Salvatore</searchLink><br /><searchLink fieldCode="AR" term="%22Roggio%2C+Tonina%22">Roggio, Tonina</searchLink><br /><searchLink fieldCode="AR" term="%22Uzzau%2C+Sergio%22">Uzzau, Sergio</searchLink><br /><searchLink fieldCode="AR" term="%22Pagnozzi%2C+Daniela%22">Pagnozzi, Daniela</searchLink><i> pagnozzi@portocontericerche.it</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Proteomics%22">Journal of Proteomics</searchLink>. Dec2011, Vol. 75 Issue 1, p93-99. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Serum%22">Serum</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Pathological+physiology%22">Pathological physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Ultrafiltration%22">Ultrafiltration</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Polyacrylamide+gel+electrophoresis%22">Polyacrylamide gel electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink>
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  Label: Abstract
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  Data: Abstract: Characterisation of blood serum peptides can provide valuable information on physiological and pathological processes. However, the analysis of raw serum samples by MS results in the identification of a limited number of peptides. In order to improve sensitivity, many peptide enrichment methods have been proposed during the last ten years. Here, we present a comparison of fractionation methods aimed to simplify analysis of small proteins and peptides in blood serum, one of the most promising sources of putative biomarkers. Specifically, three methods based on ultrafiltration, differential precipitation, and peptide ligand libraries (ProteoMiner) were evaluated for the enrichment of peptides and low molecular weight proteins, as demonstrated by Tricine SDS-PAGE and subsequent LC-MS/MS (GeLC-MS/MS). As a result, differential solubilisation (DS) allowed the identification of the highest number of peptides. Moreover, the DS method enabled also the quantitative comparison of samples, producing fundamental information in biomarker discovery approaches. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.jprot.2011.06.025
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      – Code: eng
        Text: English
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      – SubjectFull: Serum
        Type: general
      – SubjectFull: Peptides
        Type: general
      – SubjectFull: Pathological physiology
        Type: general
      – SubjectFull: Ultrafiltration
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      – SubjectFull: Biomarkers
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      – SubjectFull: Polyacrylamide gel electrophoresis
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      – SubjectFull: Mass spectrometry
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      – TitleFull: Comparison of blood serum peptide enrichment methods by Tricine SDS-PAGE and mass spectrometry
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              Text: Dec2011
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              Y: 2011
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