View in EDS

Characterization of flavonol glycosides in individual Arabidopsis root tips by flow injection electrospray mass spectrometry

Saved in:
Bibliographic Details
Title: Characterization of flavonol glycosides in individual Arabidopsis root tips by flow injection electrospray mass spectrometry
Authors: Vallabhaneni, Prashanthi1, Keith Ray, W.2, Winkel, Brenda S.J.1 winkel@vt.edu, Helm, Richard F.2
Source: Phytochemistry. Jan2012, Vol. 73, p114-118. 5p.
Subjects: Flavonol glycosides, Arabidopsis, Mass spectrometry, Plant metabolism, Quantitative research, Seedlings
Abstract: Abstract: Developments in mass spectrometry-based technologies are offering insights into the complexity and dynamic nature of plant metabolism. However, the ability to generate reliable metabolic profiles at high spatial resolution is still limited by the need of most technologies for large sample sizes or time-intensive extraction and detection methods. Here we describe the use of flow injection electrospray mass spectrometry for the rapid identification and semi-quantitative analysis of flavonol glycosides in individual root tips. This method uncovered spatial and temporal differences in metabolic profiles that were masked in analyses of whole roots or seedlings, while showing that individual biological replicates can be extremely consistent. [Copyright &y& Elsevier]
Copyright of Phytochemistry is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 70153090
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Characterization of flavonol glycosides in individual Arabidopsis root tips by flow injection electrospray mass spectrometry
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Vallabhaneni%2C+Prashanthi%22">Vallabhaneni, Prashanthi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Keith+Ray%2C+W%2E%22">Keith Ray, W.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Winkel%2C+Brenda+S%2EJ%2E%22">Winkel, Brenda S.J.</searchLink><relatesTo>1</relatesTo><i> winkel@vt.edu</i><br /><searchLink fieldCode="AR" term="%22Helm%2C+Richard+F%2E%22">Helm, Richard F.</searchLink><relatesTo>2</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Phytochemistry%22">Phytochemistry</searchLink>. Jan2012, Vol. 73, p114-118. 5p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Flavonol+glycosides%22">Flavonol glycosides</searchLink><br /><searchLink fieldCode="DE" term="%22Arabidopsis%22">Arabidopsis</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+metabolism%22">Plant metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Quantitative+research%22">Quantitative research</searchLink><br /><searchLink fieldCode="DE" term="%22Seedlings%22">Seedlings</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Developments in mass spectrometry-based technologies are offering insights into the complexity and dynamic nature of plant metabolism. However, the ability to generate reliable metabolic profiles at high spatial resolution is still limited by the need of most technologies for large sample sizes or time-intensive extraction and detection methods. Here we describe the use of flow injection electrospray mass spectrometry for the rapid identification and semi-quantitative analysis of flavonol glycosides in individual root tips. This method uncovered spatial and temporal differences in metabolic profiles that were masked in analyses of whole roots or seedlings, while showing that individual biological replicates can be extremely consistent. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Phytochemistry is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=70153090
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.phytochem.2011.09.013
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 5
        StartPage: 114
    Subjects:
      – SubjectFull: Flavonol glycosides
        Type: general
      – SubjectFull: Arabidopsis
        Type: general
      – SubjectFull: Mass spectrometry
        Type: general
      – SubjectFull: Plant metabolism
        Type: general
      – SubjectFull: Quantitative research
        Type: general
      – SubjectFull: Seedlings
        Type: general
    Titles:
      – TitleFull: Characterization of flavonol glycosides in individual Arabidopsis root tips by flow injection electrospray mass spectrometry
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Vallabhaneni, Prashanthi
      – PersonEntity:
          Name:
            NameFull: Keith Ray, W.
      – PersonEntity:
          Name:
            NameFull: Winkel, Brenda S.J.
      – PersonEntity:
          Name:
            NameFull: Helm, Richard F.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Text: Jan2012
              Type: published
              Y: 2012
          Identifiers:
            – Type: issn-print
              Value: 00319422
          Numbering:
            – Type: volume
              Value: 73
          Titles:
            – TitleFull: Phytochemistry
              Type: main
ResultId 1