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Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes

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Title: Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes
Authors: Delabona, Priscila da Silva1,2, Pirota, Rosangela D.P. Buzon1,2, Codima, Carla Aloia1,2, Tremacoldi, Célia Regina3, Rodrigues, André4, Farinas, Cristiane Sanchez2 cristiane@cnpdia.embrapa.br
Source: Biomass & Bioenergy. Feb2012, Vol. 37, p243-250. 8p.
Subjects: Microorganisms, Fermentation, Forest fungi, Xylanases, Aspergillus fumigatus, Bagasse, Glucosidases, Biomass energy
Abstract: Abstract: The successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, β-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g−1 for CMCase, 5.0 FPU g−1 for FPAse, 105.82 IU g−1 for β-glucosidase and 1055.62 IU g−1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 °C or pH 3–3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion. [Copyright &y& Elsevier]
Copyright of Biomass & Bioenergy is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 71408628
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PubTypeId: academicJournal
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  Data: Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes
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  Data: <searchLink fieldCode="AR" term="%22Delabona%2C+Priscila+da+Silva%22">Delabona, Priscila da Silva</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Pirota%2C+Rosangela+D%2EP%2E+Buzon%22">Pirota, Rosangela D.P. Buzon</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Codima%2C+Carla+Aloia%22">Codima, Carla Aloia</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Tremacoldi%2C+Célia+Regina%22">Tremacoldi, Célia Regina</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Rodrigues%2C+André%22">Rodrigues, André</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Farinas%2C+Cristiane+Sanchez%22">Farinas, Cristiane Sanchez</searchLink><relatesTo>2</relatesTo><i> cristiane@cnpdia.embrapa.br</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomass+%26+Bioenergy%22">Biomass & Bioenergy</searchLink>. Feb2012, Vol. 37, p243-250. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Microorganisms%22">Microorganisms</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Forest+fungi%22">Forest fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Xylanases%22">Xylanases</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus+fumigatus%22">Aspergillus fumigatus</searchLink><br /><searchLink fieldCode="DE" term="%22Bagasse%22">Bagasse</searchLink><br /><searchLink fieldCode="DE" term="%22Glucosidases%22">Glucosidases</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass+energy%22">Biomass energy</searchLink>
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  Data: Abstract: The successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, β-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g−1 for CMCase, 5.0 FPU g−1 for FPAse, 105.82 IU g−1 for β-glucosidase and 1055.62 IU g−1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 °C or pH 3–3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Biomass & Bioenergy is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.biombioe.2011.12.006
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      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 243
    Subjects:
      – SubjectFull: Microorganisms
        Type: general
      – SubjectFull: Fermentation
        Type: general
      – SubjectFull: Forest fungi
        Type: general
      – SubjectFull: Xylanases
        Type: general
      – SubjectFull: Aspergillus fumigatus
        Type: general
      – SubjectFull: Bagasse
        Type: general
      – SubjectFull: Glucosidases
        Type: general
      – SubjectFull: Biomass energy
        Type: general
    Titles:
      – TitleFull: Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes
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            NameFull: Delabona, Priscila da Silva
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            NameFull: Pirota, Rosangela D.P. Buzon
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            NameFull: Codima, Carla Aloia
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            NameFull: Tremacoldi, Célia Regina
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            NameFull: Rodrigues, André
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            NameFull: Farinas, Cristiane Sanchez
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              M: 02
              Text: Feb2012
              Type: published
              Y: 2012
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              Value: 09619534
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              Value: 37
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