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Stability of lactobacilli encapsulated in various microbial polymers

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Title: Stability of lactobacilli encapsulated in various microbial polymers
Authors: Jiménez-Pranteda, María Luján1,2,3 jimepra@ugr.es, Poncelet, Denis2, Náder-Macías, María Elena4, Arcos, Antonio5, Aguilera, Margarita1,3, Monteoliva-Sánchez, Mercedes1,3, Ramos-Cormenzana, Alberto1,3
Source: Journal of Bioscience & Bioengineering. Feb2012, Vol. 113 Issue 2, p179-184. 6p.
Subjects: Lactobacillus plantarum, Microbial polymers, Artificial cells, Microencapsulation, Probiotics, Suspensions (Chemistry), Bile, Hydrogen-ion concentration measurement, Scanning electron microscopy
Abstract: Abstract: Various microbial polymers, namely xanthan gum, gellan gum, pullulan gum and jamilan, were tested as a suitable encapsulating material for Lactobacillus plantarum CRL 1815 and Lactobacillus rhamnosus ATCC 53103. Resulting capsules were also studied for their pH and simulated gastrointestinal conditions tolerance. The morphology of the microcapsules was studied using scanning electron microscopy. pH tolerance was tested at pH 2.0, 3.5, 5.0 and 6.5 over a 6h incubation period. Simulated gastrointestinal conditions were assayed with simulated gastric and pancreatic juices and simulated bile over a 24h incubation period. Suspensions of probiotic organisms were used as a control. The results from encapsulation with microbial polymers indicate that mixtures of 1% xanthan gum with 0.75% gellan gum and 1% jamilan with 1% gellan gum were the most suitable for microencapsulation. Results for the pH tolerance tests showed no improvement in the viability of cells in relation to the control, except for pH 2.0 where lactobacilli encapsulated in xanthan:gellan gum (1%:0.75%) prolonged their viability by 6h exposure. Xanthan:gellan gum (1%:0.75%) was the most effective of the encapsulating materials tested in protecting L. plantarum and L. rhamnosus against simulated bile, improving its viability in 1–2logCFU when compared with control. The results of this study suggest that microbial polymers are an interesting source of encapsulating material that should be taken into account for prospective studies of probiotic encapsulation for oral delivery applications. [Copyright &y& Elsevier]
Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Stability of lactobacilli encapsulated in various microbial polymers
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  Data: <searchLink fieldCode="DE" term="%22Lactobacillus+plantarum%22">Lactobacillus plantarum</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+polymers%22">Microbial polymers</searchLink><br /><searchLink fieldCode="DE" term="%22Artificial+cells%22">Artificial cells</searchLink><br /><searchLink fieldCode="DE" term="%22Microencapsulation%22">Microencapsulation</searchLink><br /><searchLink fieldCode="DE" term="%22Probiotics%22">Probiotics</searchLink><br /><searchLink fieldCode="DE" term="%22Suspensions+%28Chemistry%29%22">Suspensions (Chemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Bile%22">Bile</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrogen-ion+concentration+measurement%22">Hydrogen-ion concentration measurement</searchLink><br /><searchLink fieldCode="DE" term="%22Scanning+electron+microscopy%22">Scanning electron microscopy</searchLink>
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  Label: Abstract
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  Data: Abstract: Various microbial polymers, namely xanthan gum, gellan gum, pullulan gum and jamilan, were tested as a suitable encapsulating material for Lactobacillus plantarum CRL 1815 and Lactobacillus rhamnosus ATCC 53103. Resulting capsules were also studied for their pH and simulated gastrointestinal conditions tolerance. The morphology of the microcapsules was studied using scanning electron microscopy. pH tolerance was tested at pH 2.0, 3.5, 5.0 and 6.5 over a 6h incubation period. Simulated gastrointestinal conditions were assayed with simulated gastric and pancreatic juices and simulated bile over a 24h incubation period. Suspensions of probiotic organisms were used as a control. The results from encapsulation with microbial polymers indicate that mixtures of 1% xanthan gum with 0.75% gellan gum and 1% jamilan with 1% gellan gum were the most suitable for microencapsulation. Results for the pH tolerance tests showed no improvement in the viability of cells in relation to the control, except for pH 2.0 where lactobacilli encapsulated in xanthan:gellan gum (1%:0.75%) prolonged their viability by 6h exposure. Xanthan:gellan gum (1%:0.75%) was the most effective of the encapsulating materials tested in protecting L. plantarum and L. rhamnosus against simulated bile, improving its viability in 1–2logCFU when compared with control. The results of this study suggest that microbial polymers are an interesting source of encapsulating material that should be taken into account for prospective studies of probiotic encapsulation for oral delivery applications. [Copyright &y& Elsevier]
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  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.jbiosc.2011.10.010
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        Text: English
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        PageCount: 6
        StartPage: 179
    Subjects:
      – SubjectFull: Lactobacillus plantarum
        Type: general
      – SubjectFull: Microbial polymers
        Type: general
      – SubjectFull: Artificial cells
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      – SubjectFull: Microencapsulation
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      – SubjectFull: Probiotics
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      – SubjectFull: Suspensions (Chemistry)
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      – SubjectFull: Bile
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      – SubjectFull: Hydrogen-ion concentration measurement
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      – SubjectFull: Scanning electron microscopy
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              Text: Feb2012
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              Y: 2012
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