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A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast.

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Title: A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast.
Authors: Wu, Guoqiu1 nationball@163.com, Wang, Xiyong2, Deng, Xuepeng3, Wu, Pengpeng3, Xue, Xiulei2, Yan, Xuejiao2, Wang, Xihua4 wangxihua@126.com
Source: Applied Microbiology & Biotechnology. Mar2012, Vol. 93 Issue 6, p2475-2482. 8p.
Subjects: Connective tissue development, Fibroblasts, Transforming growth factors, Escherichia coli, Phosphorylation
Abstract: This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatography. The yield scFv protein reached a purity over 95% after purification. Immunoreactivity assay demonstrated that scFv possessed a special affinity toward CTGF. RT-PCR, western blot, and immunofluorescence experiments showed that increased expression of α-smooth muscle actin induced by TGF-β1 could be suppressed by this scFv antibody through inhibiting the phosphorylation of Akt. [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast.
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  Data: <searchLink fieldCode="AR" term="%22Wu%2C+Guoqiu%22">Wu, Guoqiu</searchLink><relatesTo>1</relatesTo><i> nationball@163.com</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Xiyong%22">Wang, Xiyong</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Deng%2C+Xuepeng%22">Deng, Xuepeng</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Pengpeng%22">Wu, Pengpeng</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Xue%2C+Xiulei%22">Xue, Xiulei</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Yan%2C+Xuejiao%22">Yan, Xuejiao</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Xihua%22">Wang, Xihua</searchLink><relatesTo>4</relatesTo><i> wangxihua@126.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Mar2012, Vol. 93 Issue 6, p2475-2482. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Connective+tissue+development%22">Connective tissue development</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Transforming+growth+factors%22">Transforming growth factors</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphorylation%22">Phosphorylation</searchLink>
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  Data: This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatography. The yield scFv protein reached a purity over 95% after purification. Immunoreactivity assay demonstrated that scFv possessed a special affinity toward CTGF. RT-PCR, western blot, and immunofluorescence experiments showed that increased expression of α-smooth muscle actin induced by TGF-β1 could be suppressed by this scFv antibody through inhibiting the phosphorylation of Akt. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00253-011-3755-8
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