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Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2

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Title: Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2
Authors: Lee, Hye-Rim1, Park, Kyung Min2, Joung, Yoon Ki2, Park, Ki Dong2, Do, Sun Hee1 shdo@konkuk.ac.kr
Source: Journal of Controlled Release. May2012, Vol. 159 Issue 3, p332-337. 6p.
Subjects: Blood platelets, Cannabinoid receptors, Colloids in medicine, Tissue scaffolds, Cell differentiation, Drug delivery systems, Tissue engineering, Genetic regulation, Chondrogenesis
Abstract: Abstract: Three-dimensional scaffolds like hydrogels can be used for cell and drug delivery and have become a major research focus in tissue engineering. Presently, we investigated the regenerative potency of platelet-rich plasma (PRP) combined with a chondrocyte/hydrogel composite scaffold in the repair of articular cartilage defects using a rabbit model. Primary isolated joint chondrocytes from the trachlear groove of rabbit were cultured in hydrogels as follows; hydrogel (2900Pa or 5900Pa)+chondrocytes and hydrogel+chondrocytes+PRP for in vitro analysis and in vivo implantation. The 5900Pa hydrogel markedly increased cellular viability and development in a time-dependent manner. Furthermore, the hydrogels attenuated the expression of SOX-9, aggrecan, and type II collagen. PRP-containing hydrogels produced an immediate increase in mRNA levels of cannabinoid receptor (CB)1 and CB2, compared with control and PRP-free hydrogels. Osteochondral defects were enhanced recovery with formation of cartilage and perichondrium in the 5900Pa hydrogel+chondrocytes+PRP. Hydrogel may provide a suitable environment for proliferation and maturation of joint chondrocytes in relation to the gelation density and bioactive sources like PRP resulting in improvement for cartilage regeneration. [Copyright &y& Elsevier]
Copyright of Journal of Controlled Release is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 74988846
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2
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  Data: <searchLink fieldCode="AR" term="%22Lee%2C+Hye-Rim%22">Lee, Hye-Rim</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Park%2C+Kyung+Min%22">Park, Kyung Min</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Joung%2C+Yoon+Ki%22">Joung, Yoon Ki</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Park%2C+Ki+Dong%22">Park, Ki Dong</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Do%2C+Sun+Hee%22">Do, Sun Hee</searchLink><relatesTo>1</relatesTo><i> shdo@konkuk.ac.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Controlled+Release%22">Journal of Controlled Release</searchLink>. May2012, Vol. 159 Issue 3, p332-337. 6p.
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  Data: <searchLink fieldCode="DE" term="%22Blood+platelets%22">Blood platelets</searchLink><br /><searchLink fieldCode="DE" term="%22Cannabinoid+receptors%22">Cannabinoid receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Colloids+in+medicine%22">Colloids in medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+scaffolds%22">Tissue scaffolds</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+differentiation%22">Cell differentiation</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+delivery+systems%22">Drug delivery systems</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+engineering%22">Tissue engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Chondrogenesis%22">Chondrogenesis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Three-dimensional scaffolds like hydrogels can be used for cell and drug delivery and have become a major research focus in tissue engineering. Presently, we investigated the regenerative potency of platelet-rich plasma (PRP) combined with a chondrocyte/hydrogel composite scaffold in the repair of articular cartilage defects using a rabbit model. Primary isolated joint chondrocytes from the trachlear groove of rabbit were cultured in hydrogels as follows; hydrogel (2900Pa or 5900Pa)+chondrocytes and hydrogel+chondrocytes+PRP for in vitro analysis and in vivo implantation. The 5900Pa hydrogel markedly increased cellular viability and development in a time-dependent manner. Furthermore, the hydrogels attenuated the expression of SOX-9, aggrecan, and type II collagen. PRP-containing hydrogels produced an immediate increase in mRNA levels of cannabinoid receptor (CB)1 and CB2, compared with control and PRP-free hydrogels. Osteochondral defects were enhanced recovery with formation of cartilage and perichondrium in the 5900Pa hydrogel+chondrocytes+PRP. Hydrogel may provide a suitable environment for proliferation and maturation of joint chondrocytes in relation to the gelation density and bioactive sources like PRP resulting in improvement for cartilage regeneration. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Controlled Release is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jconrel.2012.02.008
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      – Code: eng
        Text: English
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        PageCount: 6
        StartPage: 332
    Subjects:
      – SubjectFull: Blood platelets
        Type: general
      – SubjectFull: Cannabinoid receptors
        Type: general
      – SubjectFull: Colloids in medicine
        Type: general
      – SubjectFull: Tissue scaffolds
        Type: general
      – SubjectFull: Cell differentiation
        Type: general
      – SubjectFull: Drug delivery systems
        Type: general
      – SubjectFull: Tissue engineering
        Type: general
      – SubjectFull: Genetic regulation
        Type: general
      – SubjectFull: Chondrogenesis
        Type: general
    Titles:
      – TitleFull: Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2
        Type: main
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            NameFull: Lee, Hye-Rim
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            NameFull: Park, Kyung Min
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            NameFull: Joung, Yoon Ki
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            NameFull: Park, Ki Dong
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            NameFull: Do, Sun Hee
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              M: 05
              Text: May2012
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              Y: 2012
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              Value: 159
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              Value: 3
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            – TitleFull: Journal of Controlled Release
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