Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues
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| Title: | Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues |
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| Authors: | Fezza, Filomena1, Bisogno, Tiziana1, Minassi, Alberto2, Appendino, Giovanni2, Mechoulam, Raphael3, Di Marzo, Vincenzo1 vdimarzo@icmib.na.cnr.it |
| Source: | FEBS Letters. Feb2002, Vol. 513 Issue 2/3, p294. 5p. |
| Subjects: | Gliomas, Thin layer chromatography, Biosynthesis |
| Abstract: | The occurrence of the novel proposed endocannabinoid, noladin ether (2-arachidonyl glyceryl ether, 2-AGE) in various rat organs and brain regions, and its inactivation by intact C6 glioma cells, were studied. 2-AGE was measured by isotope dilution liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry, with a detection limit of 100 fmol. A compound with the same mass and chromatographic/chemical properties as 2-AGE was found in whole brain, with the highest amounts in the thalamus and hippocampus. Synthetic [3H]2-AGE was inactivated by intact rat C6 glioma cells by a time- and temperature-dependent process consisting of cellular uptake and partial incorporation into phospholipids. Further data suggested that 2-AGE is taken up by cells via the anandamide/2-arachidonoyl glycerol (2-AG) membrane transporter(s), and biosynthesized in a different way as compared to 2-AG. [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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