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Preparation of stable Pd nanocubes and their use in biological labeling

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Title: Preparation of stable Pd nanocubes and their use in biological labeling
Authors: Slouf, Miroslav1 slouf@imc.cas.cz, Hruby, Martin1, Bakaeva, Zulfiya1, Vlkova, Helena1, Nebesarova, Jana2, Philimonenko, Anatoly A.3, Hozak, Pavel3
Source: Colloids & Surfaces B: Biointerfaces. Dec2012, Vol. 100, p205-208. 4p.
Subjects: Palladium compounds, Particle size distribution, Chemical reduction, Stable isotopes, Transmission electron microscopy, Gold nanoparticles
Abstract: Abstract: Stable Pd nanocubes (PdNC) with the average size ∼15nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres. [Copyright &y& Elsevier]
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Database: Engineering Source
Description
Abstract:Abstract: Stable Pd nanocubes (PdNC) with the average size ∼15nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres. [Copyright &y& Elsevier]
ISSN:09277765
DOI:10.1016/j.colsurfb.2012.05.023