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Preparation of stable Pd nanocubes and their use in biological labeling

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Title: Preparation of stable Pd nanocubes and their use in biological labeling
Authors: Slouf, Miroslav1 slouf@imc.cas.cz, Hruby, Martin1, Bakaeva, Zulfiya1, Vlkova, Helena1, Nebesarova, Jana2, Philimonenko, Anatoly A.3, Hozak, Pavel3
Source: Colloids & Surfaces B: Biointerfaces. Dec2012, Vol. 100, p205-208. 4p.
Subjects: Palladium compounds, Particle size distribution, Chemical reduction, Stable isotopes, Transmission electron microscopy, Gold nanoparticles
Abstract: Abstract: Stable Pd nanocubes (PdNC) with the average size ∼15nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres. [Copyright &y& Elsevier]
Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 78032373
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Preparation of stable Pd nanocubes and their use in biological labeling
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  Data: <searchLink fieldCode="AR" term="%22Slouf%2C+Miroslav%22">Slouf, Miroslav</searchLink><relatesTo>1</relatesTo><i> slouf@imc.cas.cz</i><br /><searchLink fieldCode="AR" term="%22Hruby%2C+Martin%22">Hruby, Martin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bakaeva%2C+Zulfiya%22">Bakaeva, Zulfiya</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Vlkova%2C+Helena%22">Vlkova, Helena</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nebesarova%2C+Jana%22">Nebesarova, Jana</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Philimonenko%2C+Anatoly+A%2E%22">Philimonenko, Anatoly A.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Hozak%2C+Pavel%22">Hozak, Pavel</searchLink><relatesTo>3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Colloids+%26+Surfaces+B%3A+Biointerfaces%22">Colloids & Surfaces B: Biointerfaces</searchLink>. Dec2012, Vol. 100, p205-208. 4p.
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  Data: <searchLink fieldCode="DE" term="%22Palladium+compounds%22">Palladium compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Particle+size+distribution%22">Particle size distribution</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+reduction%22">Chemical reduction</searchLink><br /><searchLink fieldCode="DE" term="%22Stable+isotopes%22">Stable isotopes</searchLink><br /><searchLink fieldCode="DE" term="%22Transmission+electron+microscopy%22">Transmission electron microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink>
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  Data: Abstract: Stable Pd nanocubes (PdNC) with the average size ∼15nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.colsurfb.2012.05.023
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        Text: English
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        PageCount: 4
        StartPage: 205
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        Type: general
      – SubjectFull: Particle size distribution
        Type: general
      – SubjectFull: Chemical reduction
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      – SubjectFull: Stable isotopes
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      – SubjectFull: Transmission electron microscopy
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      – SubjectFull: Gold nanoparticles
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      – TitleFull: Preparation of stable Pd nanocubes and their use in biological labeling
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              M: 12
              Text: Dec2012
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