Use of the advanced backcross-QTL method to transfer seed mineral accumulation nutrition traits from wild to Andean cultivated common beans.
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| Title: | Use of the advanced backcross-QTL method to transfer seed mineral accumulation nutrition traits from wild to Andean cultivated common beans. |
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| Authors: | Blair, Matthew mwb1@cornell.edu, Izquierdo, Paulo1 |
| Source: | Theoretical & Applied Genetics. Sep2012, Vol. 125 Issue 5, p1015-1031. 17p. |
| Subjects: | Mineral content of plants, Locus (Genetics), Cultivated plants, Common bean, Plant nutrition, Iron deficiency anemia, Zinc deficiency diseases, Plant breeding |
| Abstract: | Iron deficiency anemia and zinc deficiency are major health concerns across the world and can be addressed by biofortification breeding of higher mineral concentration in staple crops, such as common bean. Wild common beans have for the most part had higher average seed mineral concentration than cultivars of this species but have small un-commercial seeds. A logical approach for the transfer of the seed mineral trait from wild beans to cultivated beans is through the advanced backcross breeding approach. The goal of this study was to analyze a population of 138 BCF introgression lines derived from the very high iron wild genotype G10022 backcrossed into the genetic background of the commercial-type variety 'Cerinza', a large-red seeded bush bean cultivar of the Andean genepool. In addition to measuring seed mineral accumulation traits and the quantitative trait loci (QTL) controlling these traits we were interested in simultaneously testing the adaptation of the introgression lines in two replicated yield trials. We found the cross to have high polymorphism and constructed an anchored microsatellite map for the population that was 1,554-cM long and covered all 11 linkage groups of the common bean genome. Through composite interval mapping (CIM) and single point analysis (SPA), we identified associations of markers and mineral traits on b01, b06, b07, b08, b10 and b11 for seed iron concentration, and markers on b01, b04 and b10 for seed zinc concentration. The b07 and b08 QTL aligned with previous QTL for iron concentration. A large number of QTL were found for seed weight (9 with CIM and 36 with SPA analysis) and correlations between seed size and mineral content affected the identification of iron and zinc contents' QTL on many linkage groups. Segregation distortion around domestication genes made some areas difficult to introgress. However, in conclusion, the advanced backcross program produced some introgression lines with high mineral accumulation traits using a wild donor parent. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Use of the advanced backcross-QTL method to transfer seed mineral accumulation nutrition traits from wild to Andean cultivated common beans. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Blair%2C+Matthew%22">Blair, Matthew</searchLink><i> mwb1@cornell.edu</i><br /><searchLink fieldCode="AR" term="%22Izquierdo%2C+Paulo%22">Izquierdo, Paulo</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Sep2012, Vol. 125 Issue 5, p1015-1031. 17p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Mineral+content+of+plants%22">Mineral content of plants</searchLink><br /><searchLink fieldCode="DE" term="%22Locus+%28Genetics%29%22">Locus (Genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Cultivated+plants%22">Cultivated plants</searchLink><br /><searchLink fieldCode="DE" term="%22Common+bean%22">Common bean</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+nutrition%22">Plant nutrition</searchLink><br /><searchLink fieldCode="DE" term="%22Iron+deficiency+anemia%22">Iron deficiency anemia</searchLink><br /><searchLink fieldCode="DE" term="%22Zinc+deficiency+diseases%22">Zinc deficiency diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+breeding%22">Plant breeding</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Iron deficiency anemia and zinc deficiency are major health concerns across the world and can be addressed by biofortification breeding of higher mineral concentration in staple crops, such as common bean. Wild common beans have for the most part had higher average seed mineral concentration than cultivars of this species but have small un-commercial seeds. A logical approach for the transfer of the seed mineral trait from wild beans to cultivated beans is through the advanced backcross breeding approach. The goal of this study was to analyze a population of 138 BCF introgression lines derived from the very high iron wild genotype G10022 backcrossed into the genetic background of the commercial-type variety 'Cerinza', a large-red seeded bush bean cultivar of the Andean genepool. In addition to measuring seed mineral accumulation traits and the quantitative trait loci (QTL) controlling these traits we were interested in simultaneously testing the adaptation of the introgression lines in two replicated yield trials. We found the cross to have high polymorphism and constructed an anchored microsatellite map for the population that was 1,554-cM long and covered all 11 linkage groups of the common bean genome. Through composite interval mapping (CIM) and single point analysis (SPA), we identified associations of markers and mineral traits on b01, b06, b07, b08, b10 and b11 for seed iron concentration, and markers on b01, b04 and b10 for seed zinc concentration. The b07 and b08 QTL aligned with previous QTL for iron concentration. A large number of QTL were found for seed weight (9 with CIM and 36 with SPA analysis) and correlations between seed size and mineral content affected the identification of iron and zinc contents' QTL on many linkage groups. Segregation distortion around domestication genes made some areas difficult to introgress. However, in conclusion, the advanced backcross program produced some introgression lines with high mineral accumulation traits using a wild donor parent. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-012-1891-x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 1015 Subjects: – SubjectFull: Mineral content of plants Type: general – SubjectFull: Locus (Genetics) Type: general – SubjectFull: Cultivated plants Type: general – SubjectFull: Common bean Type: general – SubjectFull: Plant nutrition Type: general – SubjectFull: Iron deficiency anemia Type: general – SubjectFull: Zinc deficiency diseases Type: general – SubjectFull: Plant breeding Type: general Titles: – TitleFull: Use of the advanced backcross-QTL method to transfer seed mineral accumulation nutrition traits from wild to Andean cultivated common beans. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Blair, Matthew – PersonEntity: Name: NameFull: Izquierdo, Paulo IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2012 Type: published Y: 2012 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 125 – Type: issue Value: 5 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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