Determination of hexafluoroisopropanol, a sevoflurane urinary metabolite, by 9-fluorenylmethyl chloroformate derivatization
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| Title: | Determination of hexafluoroisopropanol, a sevoflurane urinary metabolite, by 9-fluorenylmethyl chloroformate derivatization |
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| Authors: | Buratti, M. marina.buratti@unimi.it, Valla, C., Xaiz, D., Brambilla, G., Colombi, A. |
| Source: | Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Sep2002, Vol. 776 Issue 2, p237. 7p. |
| Subjects: | High performance liquid chromatography, Urinalysis, Glucuronic acid, Hydrolysis, Fluorescence |
| Abstract: | A reversed-phase HPLC method with fluorescence detection for the quantification of hexafluoroisopropanol (HFIP) in urine is presented. HFIP, a metabolite of the inhalation anesthetic sevoflurane, is excreted mainly in urine as glucuronic acid conjugate. After enzymatic hydrolysis of the glucuronate, primary amino groups of interferent urinary compounds are blocked by reaction with o-phthalic dicarboxaldehyde and 3-mercaptopropionic acid, followed by labeling of HFIP with 9-fluorenylmethyl chloroformate. The derivatization reaction proceeds in a water–acetonitrile (1:1) solution at room temperature with a borate buffer of pH 12.5 as a catalyst. A stable fluorescent derivative of HFIP is formed within 5 min. The HFIP–FMOC derivative is separated by reversed-phase chromatography with isocratic elution on an octadecyl silyl column (33×4.6 mm, 3 μm) and guard column (20×4.0 mm, 40 μm), at 35 °C, and detected by fluorescence detection at an excitation wavelength of 265 nm and an emission wavelength of 311 nm. The method detection limit is 40 pg, per 10-μl injection volume, corresponding to 16 μg/l of HFIP in urine. The among-series relative standard deviation is <6% at 200 μg/l (n=6). As a preliminary application, the method was used to detect HFIP concentration in the urine of two volunteers exposed for 3 h to an airborne concentration of sevoflurane in the order of 2 ppm. [Copyright &y& Elsevier] |
| Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 7849581 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Determination of hexafluoroisopropanol, a sevoflurane urinary metabolite, by 9-fluorenylmethyl chloroformate derivatization – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Buratti%2C+M%2E%22">Buratti, M.</searchLink><i> marina.buratti@unimi.it</i><br /><searchLink fieldCode="AR" term="%22Valla%2C+C%2E%22">Valla, C.</searchLink><br /><searchLink fieldCode="AR" term="%22Xaiz%2C+D%2E%22">Xaiz, D.</searchLink><br /><searchLink fieldCode="AR" term="%22Brambilla%2C+G%2E%22">Brambilla, G.</searchLink><br /><searchLink fieldCode="AR" term="%22Colombi%2C+A%2E%22">Colombi, A.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Sep2002, Vol. 776 Issue 2, p237. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Urinalysis%22">Urinalysis</searchLink><br /><searchLink fieldCode="DE" term="%22Glucuronic+acid%22">Glucuronic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrolysis%22">Hydrolysis</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence%22">Fluorescence</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A reversed-phase HPLC method with fluorescence detection for the quantification of hexafluoroisopropanol (HFIP) in urine is presented. HFIP, a metabolite of the inhalation anesthetic sevoflurane, is excreted mainly in urine as glucuronic acid conjugate. After enzymatic hydrolysis of the glucuronate, primary amino groups of interferent urinary compounds are blocked by reaction with o-phthalic dicarboxaldehyde and 3-mercaptopropionic acid, followed by labeling of HFIP with 9-fluorenylmethyl chloroformate. The derivatization reaction proceeds in a water–acetonitrile (1:1) solution at room temperature with a borate buffer of pH 12.5 as a catalyst. A stable fluorescent derivative of HFIP is formed within 5 min. The HFIP–FMOC derivative is separated by reversed-phase chromatography with isocratic elution on an octadecyl silyl column (33×4.6 mm, 3 μm) and guard column (20×4.0 mm, 40 μm), at 35 °C, and detected by fluorescence detection at an excitation wavelength of 265 nm and an emission wavelength of 311 nm. The method detection limit is 40 pg, per 10-μl injection volume, corresponding to 16 μg/l of HFIP in urine. The among-series relative standard deviation is <6% at 200 μg/l (n=6). As a preliminary application, the method was used to detect HFIP concentration in the urine of two volunteers exposed for 3 h to an airborne concentration of sevoflurane in the order of 2 ppm. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/S1570-0232(02)00310-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 237 Subjects: – SubjectFull: High performance liquid chromatography Type: general – SubjectFull: Urinalysis Type: general – SubjectFull: Glucuronic acid Type: general – SubjectFull: Hydrolysis Type: general – SubjectFull: Fluorescence Type: general Titles: – TitleFull: Determination of hexafluoroisopropanol, a sevoflurane urinary metabolite, by 9-fluorenylmethyl chloroformate derivatization Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Buratti, M. – PersonEntity: Name: NameFull: Valla, C. – PersonEntity: Name: NameFull: Xaiz, D. – PersonEntity: Name: NameFull: Brambilla, G. – PersonEntity: Name: NameFull: Colombi, A. IsPartOfRelationships: – BibEntity: Dates: – D: 05 M: 09 Text: Sep2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 15700232 Numbering: – Type: volume Value: 776 – Type: issue Value: 2 Titles: – TitleFull: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences Type: main |
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