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Innovative microwave-assisted oximation and silylation procedures for metabolomic analysis of plasma samples using gas chromatography–mass spectrometry

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Title: Innovative microwave-assisted oximation and silylation procedures for metabolomic analysis of plasma samples using gas chromatography–mass spectrometry
Authors: Hong, Zhanying1, Lin, Zebin1,2, Liu, Yue1, Tan, Guangguo1, Lou, Ziyang1, Zhu, Zhenyu1, Chai, Yifeng1 yfchai@smmu.edu.cn, Fan, Guorong1 guorfan@yahoo.com.cn, Zhang, Junping1, Zhang, Liming3
Source: Journal of Chromatography A. Sep2012, Vol. 1254, p14-22. 9p.
Subjects: Technological innovations, Oxidation, Silylation, Plasma gases, Chemical sample preparation, Gas chromatography/Mass spectrometry (GC-MS), Heating
Abstract: Abstract: Analysis of plasma metabolomic samples by gas chromatography–mass spectrometry always requires comprehensive pretreatment including oximation and silylation. Although heating block (HB) is a commonly used method, it is time consuming. This study describes an extremely time-effective microwave-assisted (MA) oximation and silylation approach for metabolomic study of plasma samples. The Box–Behnken design was employed to optimize the MA conditions by means of oximation at 65W for 100s and then silylation through 180s incubation with 230W microwave irradiation. The results showed that microwave irradiation decreased the sample preparation time from approximately 180min to 5min without loss of information for the metabolites in plasma samples. Both the HB method and the developed MA method were applied in plasma metabolomic study of sulfur mustard intoxication. Partial least-squares discriminant analysis (PLS-DA) was used to globally understand the metabolic changes, and multi-criteria assessment was used to select the most significant and reliable variables as potential biomarkers. The data obtained by the MA method were in good correlation with the HB method. Compared with HB method, the newly developed MA oximation and silylation of plasma metabolome samples was more efficient and time-effective and may prove to be an attractive alternative for high-throughput sample preparation in plasma metabolomics. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 78544365
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Innovative microwave-assisted oximation and silylation procedures for metabolomic analysis of plasma samples using gas chromatography–mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Hong%2C+Zhanying%22">Hong, Zhanying</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lin%2C+Zebin%22">Lin, Zebin</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Yue%22">Liu, Yue</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Tan%2C+Guangguo%22">Tan, Guangguo</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lou%2C+Ziyang%22">Lou, Ziyang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhu%2C+Zhenyu%22">Zhu, Zhenyu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chai%2C+Yifeng%22">Chai, Yifeng</searchLink><relatesTo>1</relatesTo><i> yfchai@smmu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Fan%2C+Guorong%22">Fan, Guorong</searchLink><relatesTo>1</relatesTo><i> guorfan@yahoo.com.cn</i><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Junping%22">Zhang, Junping</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Liming%22">Zhang, Liming</searchLink><relatesTo>3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Sep2012, Vol. 1254, p14-22. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Technological+innovations%22">Technological innovations</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidation%22">Oxidation</searchLink><br /><searchLink fieldCode="DE" term="%22Silylation%22">Silylation</searchLink><br /><searchLink fieldCode="DE" term="%22Plasma+gases%22">Plasma gases</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+sample+preparation%22">Chemical sample preparation</searchLink><br /><searchLink fieldCode="DE" term="%22Gas+chromatography%2FMass+spectrometry+%28GC-MS%29%22">Gas chromatography/Mass spectrometry (GC-MS)</searchLink><br /><searchLink fieldCode="DE" term="%22Heating%22">Heating</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Analysis of plasma metabolomic samples by gas chromatography–mass spectrometry always requires comprehensive pretreatment including oximation and silylation. Although heating block (HB) is a commonly used method, it is time consuming. This study describes an extremely time-effective microwave-assisted (MA) oximation and silylation approach for metabolomic study of plasma samples. The Box–Behnken design was employed to optimize the MA conditions by means of oximation at 65W for 100s and then silylation through 180s incubation with 230W microwave irradiation. The results showed that microwave irradiation decreased the sample preparation time from approximately 180min to 5min without loss of information for the metabolites in plasma samples. Both the HB method and the developed MA method were applied in plasma metabolomic study of sulfur mustard intoxication. Partial least-squares discriminant analysis (PLS-DA) was used to globally understand the metabolic changes, and multi-criteria assessment was used to select the most significant and reliable variables as potential biomarkers. The data obtained by the MA method were in good correlation with the HB method. Compared with HB method, the newly developed MA oximation and silylation of plasma metabolome samples was more efficient and time-effective and may prove to be an attractive alternative for high-throughput sample preparation in plasma metabolomics. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.chroma.2012.07.033
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 9
        StartPage: 14
    Subjects:
      – SubjectFull: Technological innovations
        Type: general
      – SubjectFull: Oxidation
        Type: general
      – SubjectFull: Silylation
        Type: general
      – SubjectFull: Plasma gases
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      – SubjectFull: Chemical sample preparation
        Type: general
      – SubjectFull: Gas chromatography/Mass spectrometry (GC-MS)
        Type: general
      – SubjectFull: Heating
        Type: general
    Titles:
      – TitleFull: Innovative microwave-assisted oximation and silylation procedures for metabolomic analysis of plasma samples using gas chromatography–mass spectrometry
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            NameFull: Hong, Zhanying
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            NameFull: Lin, Zebin
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            – D: 07
              M: 09
              Text: Sep2012
              Type: published
              Y: 2012
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