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Intramolecular 13C pattern in hexoses from autotrophic and heterotrophic C3 plant tissues.

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Title: Intramolecular 13C pattern in hexoses from autotrophic and heterotrophic C3 plant tissues.
Authors: Gilbert, Alexis1,2,3 gilbert.a.aa@mtitech.ac.jp, Robins, Richard J.1, Remaud, Gérald S.1, Tcherkez, Guillaume G. B.2,4
Source: Proceedings of the National Academy of Sciences of the United States of America. 10/30/2012, Vol. 109 Issue 44, p18204-18209. 6p.
Subjects: Ecophysiology, Photosynthates, Plant ecophysiology, Sucrose, Glucose, Plant cells & tissues
Abstract: The stable carbon isotope 13C is used as a universal tracer in plant eco-physiology and studies of carbon exchange between vegetation and atmosphere. Photosynthesis fractionates against 13CO2 so that source sugars (photosynthates) are on average 13C depleted by 20%o compared with atmospheric CO2. The carbon isotope distribution within sugars has been shown to be heterogeneous, with relatively 13C-enriched and 13C-depleted C-atom positions. The 13C pattern within sugars is the cornerstone of 13C distribution in plants, because all metabolites inherit the 13C abundance in their specific precursor C-atom positions. However, the intramolecular isotope pattern in source leaf glucose and the isotope fractionation associated with key enzymes involved in sugar interconversions are currently unknown. To gain insight into these, we have analyzed the intramolecular isotope composition in source leaf transient starch, grain storage starch, and root storage sucrose and measured the site-specific isotope fractionation associated with the invertase (EC 3.2.1.26) and glucose isomerase (EC 5.3.1.5) reactions. When these data are integrated into a simple steady-state model of plant isotopic fluxes, the enzyme-dependent fractionations satisfactorily predict the observed intramolecular patterns. These results demonstrate that glucose and sucrose metabolism is the primary determinant of the 13C abundance in source and sink tissue and is, therefore, of fundamental importance to the interpretation of plant isotopic signals. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 83142377
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Intramolecular <superscript>13</superscript>C pattern in hexoses from autotrophic and heterotrophic C<subscript>3</subscript> plant tissues.
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  Data: <searchLink fieldCode="DE" term="%22Ecophysiology%22">Ecophysiology</searchLink><br /><searchLink fieldCode="DE" term="%22Photosynthates%22">Photosynthates</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+ecophysiology%22">Plant ecophysiology</searchLink><br /><searchLink fieldCode="DE" term="%22Sucrose%22">Sucrose</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose%22">Glucose</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+cells+%26+tissues%22">Plant cells & tissues</searchLink>
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  Data: The stable carbon isotope 13C is used as a universal tracer in plant eco-physiology and studies of carbon exchange between vegetation and atmosphere. Photosynthesis fractionates against 13CO2 so that source sugars (photosynthates) are on average 13C depleted by 20%o compared with atmospheric CO2. The carbon isotope distribution within sugars has been shown to be heterogeneous, with relatively 13C-enriched and 13C-depleted C-atom positions. The 13C pattern within sugars is the cornerstone of 13C distribution in plants, because all metabolites inherit the 13C abundance in their specific precursor C-atom positions. However, the intramolecular isotope pattern in source leaf glucose and the isotope fractionation associated with key enzymes involved in sugar interconversions are currently unknown. To gain insight into these, we have analyzed the intramolecular isotope composition in source leaf transient starch, grain storage starch, and root storage sucrose and measured the site-specific isotope fractionation associated with the invertase (EC 3.2.1.26) and glucose isomerase (EC 5.3.1.5) reactions. When these data are integrated into a simple steady-state model of plant isotopic fluxes, the enzyme-dependent fractionations satisfactorily predict the observed intramolecular patterns. These results demonstrate that glucose and sucrose metabolism is the primary determinant of the 13C abundance in source and sink tissue and is, therefore, of fundamental importance to the interpretation of plant isotopic signals. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1073/pnas.1211149109
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        Text: English
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      – SubjectFull: Plant ecophysiology
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      – SubjectFull: Sucrose
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      – SubjectFull: Glucose
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      – SubjectFull: Plant cells & tissues
        Type: general
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      – TitleFull: Intramolecular 13C pattern in hexoses from autotrophic and heterotrophic C3 plant tissues.
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            NameFull: Gilbert, Alexis
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            NameFull: Remaud, Gérald S.
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            NameFull: Tcherkez, Guillaume G. B.
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              Text: 10/30/2012
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