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Microenvironments and different nanoparticle dynamics in living cells revealed by a standard nanoparticle

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Title: Microenvironments and different nanoparticle dynamics in living cells revealed by a standard nanoparticle
Authors: Pack, Chan Gi1 cback@riken.jp, Song, Mi Ryoung2, Lee Tae, Eunju2, Hiroshima, Michio1, Byun, Kyung Hee3, Kim, Jun Sung2 lifeisgood@biterials.com, Sako, Yasushi1
Source: Journal of Controlled Release. Nov2012, Vol. 163 Issue 3, p315-321. 7p.
Subjects: Silica nanoparticles, Nanoparticle synthesis, Molecular probes, Drug delivery systems, Light scattering, Transmission electron microscopy, Quantitative research
Abstract: Abstract: For quantitative analysis of nanoparticle diffusions and submicro-environments in living cells, use of newly synthesized silica-based fluorescent nanoparticle (Si-FNP) as a standard nanoprobe is successfully demonstrated. The appropriate characteristics of a standard probe were fully analyzed in vitro by single molecule detection, transmission electron microscopy, and dynamic light scattering. Using fluorescence correlation analysis in single living cells, we quantitatively compared the diffusional properties of the standard Si-FNP with a diameter of 50nm, peptide coated Si-FNP, streptavidin coated Qdot, and GFP molecule which have different sizes and surface properties. The result demonstrates that the standard Si-FNP without coat is minimally trapped in the vesicles in the process of cellular endocytosis. Interestingly, a large proportion of Si-FNP introduced into the cells by electroporation diffuses freely in the cells during a cell cycle suggesting free diffusing NPs are hardly trapped in the vesicles. The simple but highly sensitive method will provide insight into strategies to understanding the hydrodynamic process of nanoparticle delivery into living cells as well as the cellular microenvironment in the view of submicro-size. [Copyright &y& Elsevier]
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Database: Engineering Source
Description
Abstract:Abstract: For quantitative analysis of nanoparticle diffusions and submicro-environments in living cells, use of newly synthesized silica-based fluorescent nanoparticle (Si-FNP) as a standard nanoprobe is successfully demonstrated. The appropriate characteristics of a standard probe were fully analyzed in vitro by single molecule detection, transmission electron microscopy, and dynamic light scattering. Using fluorescence correlation analysis in single living cells, we quantitatively compared the diffusional properties of the standard Si-FNP with a diameter of 50nm, peptide coated Si-FNP, streptavidin coated Qdot, and GFP molecule which have different sizes and surface properties. The result demonstrates that the standard Si-FNP without coat is minimally trapped in the vesicles in the process of cellular endocytosis. Interestingly, a large proportion of Si-FNP introduced into the cells by electroporation diffuses freely in the cells during a cell cycle suggesting free diffusing NPs are hardly trapped in the vesicles. The simple but highly sensitive method will provide insight into strategies to understanding the hydrodynamic process of nanoparticle delivery into living cells as well as the cellular microenvironment in the view of submicro-size. [Copyright &y& Elsevier]
ISSN:01683659
DOI:10.1016/j.jconrel.2012.07.036