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Microenvironments and different nanoparticle dynamics in living cells revealed by a standard nanoparticle

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Title: Microenvironments and different nanoparticle dynamics in living cells revealed by a standard nanoparticle
Authors: Pack, Chan Gi1 cback@riken.jp, Song, Mi Ryoung2, Lee Tae, Eunju2, Hiroshima, Michio1, Byun, Kyung Hee3, Kim, Jun Sung2 lifeisgood@biterials.com, Sako, Yasushi1
Source: Journal of Controlled Release. Nov2012, Vol. 163 Issue 3, p315-321. 7p.
Subjects: Silica nanoparticles, Nanoparticle synthesis, Molecular probes, Drug delivery systems, Light scattering, Transmission electron microscopy, Quantitative research
Abstract: Abstract: For quantitative analysis of nanoparticle diffusions and submicro-environments in living cells, use of newly synthesized silica-based fluorescent nanoparticle (Si-FNP) as a standard nanoprobe is successfully demonstrated. The appropriate characteristics of a standard probe were fully analyzed in vitro by single molecule detection, transmission electron microscopy, and dynamic light scattering. Using fluorescence correlation analysis in single living cells, we quantitatively compared the diffusional properties of the standard Si-FNP with a diameter of 50nm, peptide coated Si-FNP, streptavidin coated Qdot, and GFP molecule which have different sizes and surface properties. The result demonstrates that the standard Si-FNP without coat is minimally trapped in the vesicles in the process of cellular endocytosis. Interestingly, a large proportion of Si-FNP introduced into the cells by electroporation diffuses freely in the cells during a cell cycle suggesting free diffusing NPs are hardly trapped in the vesicles. The simple but highly sensitive method will provide insight into strategies to understanding the hydrodynamic process of nanoparticle delivery into living cells as well as the cellular microenvironment in the view of submicro-size. [Copyright &y& Elsevier]
Copyright of Journal of Controlled Release is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 83296084
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Controlled+Release%22">Journal of Controlled Release</searchLink>. Nov2012, Vol. 163 Issue 3, p315-321. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Silica+nanoparticles%22">Silica nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Nanoparticle+synthesis%22">Nanoparticle synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+probes%22">Molecular probes</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+delivery+systems%22">Drug delivery systems</searchLink><br /><searchLink fieldCode="DE" term="%22Light+scattering%22">Light scattering</searchLink><br /><searchLink fieldCode="DE" term="%22Transmission+electron+microscopy%22">Transmission electron microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Quantitative+research%22">Quantitative research</searchLink>
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  Data: Abstract: For quantitative analysis of nanoparticle diffusions and submicro-environments in living cells, use of newly synthesized silica-based fluorescent nanoparticle (Si-FNP) as a standard nanoprobe is successfully demonstrated. The appropriate characteristics of a standard probe were fully analyzed in vitro by single molecule detection, transmission electron microscopy, and dynamic light scattering. Using fluorescence correlation analysis in single living cells, we quantitatively compared the diffusional properties of the standard Si-FNP with a diameter of 50nm, peptide coated Si-FNP, streptavidin coated Qdot, and GFP molecule which have different sizes and surface properties. The result demonstrates that the standard Si-FNP without coat is minimally trapped in the vesicles in the process of cellular endocytosis. Interestingly, a large proportion of Si-FNP introduced into the cells by electroporation diffuses freely in the cells during a cell cycle suggesting free diffusing NPs are hardly trapped in the vesicles. The simple but highly sensitive method will provide insight into strategies to understanding the hydrodynamic process of nanoparticle delivery into living cells as well as the cellular microenvironment in the view of submicro-size. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Journal of Controlled Release is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jconrel.2012.07.036
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 7
        StartPage: 315
    Subjects:
      – SubjectFull: Silica nanoparticles
        Type: general
      – SubjectFull: Nanoparticle synthesis
        Type: general
      – SubjectFull: Molecular probes
        Type: general
      – SubjectFull: Drug delivery systems
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      – SubjectFull: Light scattering
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      – SubjectFull: Transmission electron microscopy
        Type: general
      – SubjectFull: Quantitative research
        Type: general
    Titles:
      – TitleFull: Microenvironments and different nanoparticle dynamics in living cells revealed by a standard nanoparticle
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            NameFull: Pack, Chan Gi
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            NameFull: Song, Mi Ryoung
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            NameFull: Hiroshima, Michio
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            NameFull: Byun, Kyung Hee
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              Text: Nov2012
              Type: published
              Y: 2012
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              Value: 163
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