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Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms

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Title: Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms
Authors: Chang, Ya Hui1, Lin, Li-Mei1, Lou, Chi-Wen1, Chou, Chuan-Kai2, Ch’ang, Hui-Ju1,3 hjmc@nhri.org.tw
Source: Radiotherapy & Oncology. Dec2012, Vol. 105 Issue 3, p371-377. 7p.
Subjects: Bone marrow transplantation, Intestinal mucosa, Total body irradiation, Paracrine mechanisms, Stromal cells, Cell proliferation, Epithelial cells, Regeneration (Biology)
Abstract: Abstract: Purpose: Our previous study reveals bone marrow transplantation (BMT) recruits host marrow-derived myelomonocytic cells to radiation-injured intestine, enhancing stromal proliferation, leading secondarily to epithelial regeneration. In this study, we propose BMT ameliorates intestinal damage via paracrine mechanisms. Materials and methods: Angiogenic cytokines within the intestinal mucosa of mice after whole body irradiation (WBI) with or without BMT were measured by cytokine array and ELISA. BM conditioned medium (BMCM) with or without treatment with neutralizing antibodies to angiogenic cytokines were continuously infused into mice for three days after radiation. Carrageenan was used to deplete myelomonocytic cells of mice. Results: BMT increased VEGF, bFGF and other angiogenic and chemotactic cytokines in the intestinal mucosa within 24h after WBI. Infusion of BMCM ameliorated radiation-induced intestinal damage with improved stromal activity and prolonged survival of mice. Neutralization of bFGF, PDGF and other angiogenic cytokines within BMCM abolished the mitigating effect to the intestine. Pretreatment of carrageenan to recipient mice reversed some of the cytokine levels, including VEGF, bFGF and IGF within the intestinal mucosa after BMT. Conclusions: Our result suggests BMT recruits host myelomonocytic cells and enhances intestinal stroma proliferation after radiation by secreting cytokines enhancing angiogenesis and chemotaxis. Host myelomonocytic cells further uplift the paracrine effect to enhance intestinal mucosal recovery. [Copyright &y& Elsevier]
Copyright of Radiotherapy & Oncology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 84359661
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PubTypeId: academicJournal
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Items – Name: Title
  Label: Title
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  Data: Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms
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  Data: <searchLink fieldCode="AR" term="%22Chang%2C+Ya+Hui%22">Chang, Ya Hui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lin%2C+Li-Mei%22">Lin, Li-Mei</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lou%2C+Chi-Wen%22">Lou, Chi-Wen</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chou%2C+Chuan-Kai%22">Chou, Chuan-Kai</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ch’ang%2C+Hui-Ju%22">Ch’ang, Hui-Ju</searchLink><relatesTo>1,3</relatesTo><i> hjmc@nhri.org.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22Radiotherapy+%26+Oncology%22">Radiotherapy & Oncology</searchLink>. Dec2012, Vol. 105 Issue 3, p371-377. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Bone+marrow+transplantation%22">Bone marrow transplantation</searchLink><br /><searchLink fieldCode="DE" term="%22Intestinal+mucosa%22">Intestinal mucosa</searchLink><br /><searchLink fieldCode="DE" term="%22Total+body+irradiation%22">Total body irradiation</searchLink><br /><searchLink fieldCode="DE" term="%22Paracrine+mechanisms%22">Paracrine mechanisms</searchLink><br /><searchLink fieldCode="DE" term="%22Stromal+cells%22">Stromal cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial+cells%22">Epithelial cells</searchLink><br /><searchLink fieldCode="DE" term="%22Regeneration+%28Biology%29%22">Regeneration (Biology)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Purpose: Our previous study reveals bone marrow transplantation (BMT) recruits host marrow-derived myelomonocytic cells to radiation-injured intestine, enhancing stromal proliferation, leading secondarily to epithelial regeneration. In this study, we propose BMT ameliorates intestinal damage via paracrine mechanisms. Materials and methods: Angiogenic cytokines within the intestinal mucosa of mice after whole body irradiation (WBI) with or without BMT were measured by cytokine array and ELISA. BM conditioned medium (BMCM) with or without treatment with neutralizing antibodies to angiogenic cytokines were continuously infused into mice for three days after radiation. Carrageenan was used to deplete myelomonocytic cells of mice. Results: BMT increased VEGF, bFGF and other angiogenic and chemotactic cytokines in the intestinal mucosa within 24h after WBI. Infusion of BMCM ameliorated radiation-induced intestinal damage with improved stromal activity and prolonged survival of mice. Neutralization of bFGF, PDGF and other angiogenic cytokines within BMCM abolished the mitigating effect to the intestine. Pretreatment of carrageenan to recipient mice reversed some of the cytokine levels, including VEGF, bFGF and IGF within the intestinal mucosa after BMT. Conclusions: Our result suggests BMT recruits host myelomonocytic cells and enhances intestinal stroma proliferation after radiation by secreting cytokines enhancing angiogenesis and chemotaxis. Host myelomonocytic cells further uplift the paracrine effect to enhance intestinal mucosal recovery. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Radiotherapy & Oncology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.radonc.2012.10.005
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 371
    Subjects:
      – SubjectFull: Bone marrow transplantation
        Type: general
      – SubjectFull: Intestinal mucosa
        Type: general
      – SubjectFull: Total body irradiation
        Type: general
      – SubjectFull: Paracrine mechanisms
        Type: general
      – SubjectFull: Stromal cells
        Type: general
      – SubjectFull: Cell proliferation
        Type: general
      – SubjectFull: Epithelial cells
        Type: general
      – SubjectFull: Regeneration (Biology)
        Type: general
    Titles:
      – TitleFull: Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms
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            NameFull: Chang, Ya Hui
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            NameFull: Lin, Li-Mei
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            NameFull: Lou, Chi-Wen
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            NameFull: Chou, Chuan-Kai
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            NameFull: Ch’ang, Hui-Ju
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              M: 12
              Text: Dec2012
              Type: published
              Y: 2012
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              Value: 105
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