Biotransformation of codeine to 14-OH-codeine derivatives by Rhizobium radiobacter R89-1
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| Title: | Biotransformation of codeine to 14-OH-codeine derivatives by Rhizobium radiobacter R89-1 |
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| Authors: | Kyslíková, Eva1, Babiak, Peter1,2, Štěpánek, Václav1 stepanek@biomed.cas.cz, Zahradník, Jiří1, Palyzová, Andrea1, Marešová, Helena1, Valešová, Renáta1, Hájíček, Josef2, Kyslík, Pavel1 |
| Source: | Journal of Molecular Catalysis B: Enzymatic. Mar2013, Vol. 87, p1-5. 5p. |
| Subjects: | Biotransformation (Metabolism), Codeine derivatives, Agrobacterium radiobacter, Enzymes, Gram-negative bacteria, Opium poppy, Ribosomal RNA, Morphine |
| Abstract: | Abstract: A Gram-negative bacterium transforming codeine 1a to 14-hydroxycodeine 1c was isolated from the sample of composted wastes of Papaver somniferum deposited in a factory producing poppy seed. Based on 16S ribosomal RNA gene phylogeny, biochemical and morphological properties, the strain was designated as Rhizobium radiobacter R89-1. The bacterium is able to transform 1a into four C-14-hydroxy derivatives of codeine. When used as a whole-cell catalyst, 100% conversion was achieved at concentration of 1a up to 6.0g/L to 14-hydroxycodeine 1c as a single product. Biohydroxylation of codeine to C-14-hydroxy derivatives, a high tolerance of the strain to 1a and activity with morphine 2a make the biocatalyst promising for pharmaceutical applications. [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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