Bibliographic Details
| Title: |
Structure determination from a single high-pressure-frozen virus crystal. |
| Authors: |
Burkhardt, Anja, Wagner, Armin, Warmer, Martin, Reimer, Rudolph, Hohenberg, Heinrich, Ren, Jingshan, Fry, Elizabeth E., Stuart, David I., Meents, Alke |
| Source: |
Acta Crystallographica: Section D (Wiley-Blackwell). Feb2013, Vol. 69 Issue 2, p308-312. 5p. |
| Subjects: |
Bos, Molecular crystals, Crystal structure, Low temperature engineering, High pressure (Technology), Acquisition of data, Enteroviruses |
| Abstract: |
Successful cryogenic X-ray structure determination from a single high-pressure-frozen bovine enterovirus 2 crystal is reported. The presented high-pressure-freezing procedure is based on a commercially available device and allows the cryocooling of macromolecular crystals directly in their mother liquor without the time- and crystal-consuming search for optimal cryoconditions. The method is generally applicable and will allow cryogenic data collection from all types of macromolecular crystals. [ABSTRACT FROM AUTHOR] |
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| Database: |
Engineering Source |
