Influence of chromatographic conditions on separation in comprehensive gas chromatography
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| Title: | Influence of chromatographic conditions on separation in comprehensive gas chromatography |
|---|---|
| Authors: | Ong, Ruby, Marriott, Philip philip.marriott@rmit.edu.au, Morrison, Paul, Haglund, Peter |
| Source: | Journal of Chromatography A. Jul2002, Vol. 962 Issue 1/2, p135. 18p. |
| Subjects: | Gas chromatography, Temperature, Chromatographic analysis |
| Abstract: | Comprehensive gas chromatography (GC×GC) is now established as a powerful technique, which offers unprecedented separation power. For complex samples, the distribution of peaks in the two-dimensional (2D) space still may need to be optimised. Since temperature (T) is a critical variable, and compounds can be shifted in relative positions on column 1 arising from temperature program rate (rT) changes, and since retention in the second dimension, D2 (2tR) is likewise affected by the prevailing T (elution temperature; Te), then any factors which alter Te will affect the extent of separation in D2. Since temperature program and carrier gas velocity rate will affect the Te of the solutes, these two factors are considered in this paper. Apart from these two parameters, results of different stationary phase choice for the second dimension column as well as the second dimension column length are reported. rT is found to have the most profound impact on the Te of solutes and will be more likely to cause an inversion of elution order if such behaviour can occur. Peak widths and 2tR increase with a decrease in Te. On the other hand, flow-rate has less impact on peak widths and 2tR although Te is affected by a change in flow-rate. Specific solute–stationary phase interactions will cause the elution order of certain solutes to be altered, and may be observed when a different stationary phase is employed as the second column, depending on the solute–stationary phase separation mechanism. Experiments conducted on different second dimension column length showed that although a longer column will lead to better separations, wrap-around may confound the separation process and may cause the solutes from sequential modulation events to co-elute. Thus a suitable second dimension column phase and length must be employed in order to obtain good separation. The factors investigated in this study will cause different extents of changes in the solute elution order and solute separations, and will affect the 2D contour presentation. [Copyright &y& Elsevier] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Influence of chromatographic conditions on separation in comprehensive gas chromatography – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ong%2C+Ruby%22">Ong, Ruby</searchLink><br /><searchLink fieldCode="AR" term="%22Marriott%2C+Philip%22">Marriott, Philip</searchLink><i> philip.marriott@rmit.edu.au</i><br /><searchLink fieldCode="AR" term="%22Morrison%2C+Paul%22">Morrison, Paul</searchLink><br /><searchLink fieldCode="AR" term="%22Haglund%2C+Peter%22">Haglund, Peter</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Jul2002, Vol. 962 Issue 1/2, p135. 18p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Gas+chromatography%22">Gas chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Temperature%22">Temperature</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Comprehensive gas chromatography (GC×GC) is now established as a powerful technique, which offers unprecedented separation power. For complex samples, the distribution of peaks in the two-dimensional (2D) space still may need to be optimised. Since temperature (T) is a critical variable, and compounds can be shifted in relative positions on column 1 arising from temperature program rate (rT) changes, and since retention in the second dimension, D2 (2tR) is likewise affected by the prevailing T (elution temperature; Te), then any factors which alter Te will affect the extent of separation in D2. Since temperature program and carrier gas velocity rate will affect the Te of the solutes, these two factors are considered in this paper. Apart from these two parameters, results of different stationary phase choice for the second dimension column as well as the second dimension column length are reported. rT is found to have the most profound impact on the Te of solutes and will be more likely to cause an inversion of elution order if such behaviour can occur. Peak widths and 2tR increase with a decrease in Te. On the other hand, flow-rate has less impact on peak widths and 2tR although Te is affected by a change in flow-rate. Specific solute–stationary phase interactions will cause the elution order of certain solutes to be altered, and may be observed when a different stationary phase is employed as the second column, depending on the solute–stationary phase separation mechanism. Experiments conducted on different second dimension column length showed that although a longer column will lead to better separations, wrap-around may confound the separation process and may cause the solutes from sequential modulation events to co-elute. Thus a suitable second dimension column phase and length must be employed in order to obtain good separation. The factors investigated in this study will cause different extents of changes in the solute elution order and solute separations, and will affect the 2D contour presentation. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/S0021-9673(02)00458-2 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 18 StartPage: 135 Subjects: – SubjectFull: Gas chromatography Type: general – SubjectFull: Temperature Type: general – SubjectFull: Chromatographic analysis Type: general Titles: – TitleFull: Influence of chromatographic conditions on separation in comprehensive gas chromatography Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ong, Ruby – PersonEntity: Name: NameFull: Marriott, Philip – PersonEntity: Name: NameFull: Morrison, Paul – PersonEntity: Name: NameFull: Haglund, Peter IsPartOfRelationships: – BibEntity: Dates: – D: 12 M: 07 Text: Jul2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 962 – Type: issue Value: 1/2 Titles: – TitleFull: Journal of Chromatography A Type: main |
| ResultId | 1 |
