The Role of Drosophila Heparan Sulfate 6-O-Endosulfatase in Sulfation Compensation.
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| Title: | The Role of Drosophila Heparan Sulfate 6-O-Endosulfatase in Sulfation Compensation. |
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| Authors: | Dejima, Katsufumi1, Kleinschmit, Adam1, Takemura, Masahiko1, Pui Yee Choi1, Kinoshita-Toyoda, Akiko2, Toyoda, Hidenao2, Nakato, Hiroshi1 nakat003@umn.edu |
| Source: | Journal of Biological Chemistry. 3/1/2013, Vol. 288 Issue 9, p6574-6582. 9p. |
| Subjects: | Drosophila, Heparan sulfate proteoglycans, Biosynthesis, Sulfation, Proteoglycans, Decapentaplegic protein, Hedgehog signaling proteins |
| Abstract: | The biosynthesis of heparan sulfate proteoglycans is tightly regulated by multiple feedback mechanisms, which support robust developmental systems. One of the regulatory network systems controlling heparan sulfate (HS) biosynthesis is sulfation compensation. A previous study using Drosophila HS 2-O- and 6-O-sulfotransferase (Hs2st and Hs6st) mutants showed that loss of sulfation at one position is compensated by increased sulfation at other positions, supporting normal FGF signaling. Here, we show that HS sulfation compensation rescues both Decapentaplegic and Wingless signaling, suggesting a universal role of this regulatory system in multiple pathways in Drosophila. Furthermore, we identified Sulf1, extracellularHS6-O-endosulfatase, as a novel component of HS sulfation compensation. Simultaneous loss of Hs2st and Sulf1 led to 6-O-oversulfation, leading to patterning defects, overgrowth, and lethality. These phenotypes are caused at least partly by abnormal up-regulation of Hedgehog signaling. Thus, sulfation compensation depends on the coordinated activities of Hs2st, Hs6st, and Sulf1. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 85989190 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The Role of Drosophila Heparan Sulfate 6-O-Endosulfatase in Sulfation Compensation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dejima%2C+Katsufumi%22">Dejima, Katsufumi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kleinschmit%2C+Adam%22">Kleinschmit, Adam</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Takemura%2C+Masahiko%22">Takemura, Masahiko</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pui+Yee+Choi%22">Pui Yee Choi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kinoshita-Toyoda%2C+Akiko%22">Kinoshita-Toyoda, Akiko</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Toyoda%2C+Hidenao%22">Toyoda, Hidenao</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Nakato%2C+Hiroshi%22">Nakato, Hiroshi</searchLink><relatesTo>1</relatesTo><i> nakat003@umn.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 3/1/2013, Vol. 288 Issue 9, p6574-6582. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Drosophila%22">Drosophila</searchLink><br /><searchLink fieldCode="DE" term="%22Heparan+sulfate+proteoglycans%22">Heparan sulfate proteoglycans</searchLink><br /><searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Sulfation%22">Sulfation</searchLink><br /><searchLink fieldCode="DE" term="%22Proteoglycans%22">Proteoglycans</searchLink><br /><searchLink fieldCode="DE" term="%22Decapentaplegic+protein%22">Decapentaplegic protein</searchLink><br /><searchLink fieldCode="DE" term="%22Hedgehog+signaling+proteins%22">Hedgehog signaling proteins</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The biosynthesis of heparan sulfate proteoglycans is tightly regulated by multiple feedback mechanisms, which support robust developmental systems. One of the regulatory network systems controlling heparan sulfate (HS) biosynthesis is sulfation compensation. A previous study using Drosophila HS 2-O- and 6-O-sulfotransferase (Hs2st and Hs6st) mutants showed that loss of sulfation at one position is compensated by increased sulfation at other positions, supporting normal FGF signaling. Here, we show that HS sulfation compensation rescues both Decapentaplegic and Wingless signaling, suggesting a universal role of this regulatory system in multiple pathways in Drosophila. Furthermore, we identified Sulf1, extracellularHS6-O-endosulfatase, as a novel component of HS sulfation compensation. Simultaneous loss of Hs2st and Sulf1 led to 6-O-oversulfation, leading to patterning defects, overgrowth, and lethality. These phenotypes are caused at least partly by abnormal up-regulation of Hedgehog signaling. Thus, sulfation compensation depends on the coordinated activities of Hs2st, Hs6st, and Sulf1. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1074/jbc.M112.404830 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 6574 Subjects: – SubjectFull: Drosophila Type: general – SubjectFull: Heparan sulfate proteoglycans Type: general – SubjectFull: Biosynthesis Type: general – SubjectFull: Sulfation Type: general – SubjectFull: Proteoglycans Type: general – SubjectFull: Decapentaplegic protein Type: general – SubjectFull: Hedgehog signaling proteins Type: general Titles: – TitleFull: The Role of Drosophila Heparan Sulfate 6-O-Endosulfatase in Sulfation Compensation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dejima, Katsufumi – PersonEntity: Name: NameFull: Kleinschmit, Adam – PersonEntity: Name: NameFull: Takemura, Masahiko – PersonEntity: Name: NameFull: Pui Yee Choi – PersonEntity: Name: NameFull: Kinoshita-Toyoda, Akiko – PersonEntity: Name: NameFull: Toyoda, Hidenao – PersonEntity: Name: NameFull: Nakato, Hiroshi IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: 3/1/2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 00219258 Numbering: – Type: volume Value: 288 – Type: issue Value: 9 Titles: – TitleFull: Journal of Biological Chemistry Type: main |
| ResultId | 1 |
