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The antagonistic roles of PDGF and integrin αvβ3 in regulating ROS production at focal adhesions

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Title: The antagonistic roles of PDGF and integrin αvβ3 in regulating ROS production at focal adhesions
Authors: Lin, Li-Jung1, Grimme, Jill M.2, Sun, Jie1, Lu, Shaoying1, Gai, Lisa3, Cropek, Donald M.2 Donald.M.Cropek@usace.army.mil, Wang, Yingxiao1,4,5,6 yiw015@eng.ucsd.edu
Source: Biomaterials. May2013, Vol. 34 Issue 15, p3807-3815. 9p.
Subjects: Platelet-derived growth factor, Integrins, Reactive oxygen species, Focal adhesions, Cell migration, Fluorescence resonance energy transfer
Abstract: Abstract: Reactive oxygen species (ROS) have been shown to play crucial roles in regulating various cellular functions, e.g. focal adhesion (FA) dynamics and cell migration upon growth factor stimulation. However, it is not clear how ROS are regulated at subcellular FA sites to impact cell migration. We have developed a biosensor capable of monitoring ROS production at FA sites in live cells with high sensitivity and specificity, utilizing fluorescence resonance energy transfer (FRET). The results revealed that platelet derived growth factor (PDGF) can induce ROS production at FA sites, which is mediated by Rac1 activation. In contrast, integrins, specifically integrin αvβ3, inhibits this local ROS production. The RhoA activity can mediate this inhibitory role of integrins in regulating ROS production. Therefore, PDGF and integrin αvβ3 coordinate to have an antagonistic effect in the ROS production at FA sites to regulate cell adhesion and migration. [Copyright &y& Elsevier]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 86408454
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: The antagonistic roles of PDGF and integrin αvβ3 in regulating ROS production at focal adhesions
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  Data: <searchLink fieldCode="AR" term="%22Lin%2C+Li-Jung%22">Lin, Li-Jung</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Grimme%2C+Jill+M%2E%22">Grimme, Jill M.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sun%2C+Jie%22">Sun, Jie</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lu%2C+Shaoying%22">Lu, Shaoying</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gai%2C+Lisa%22">Gai, Lisa</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Cropek%2C+Donald+M%2E%22">Cropek, Donald M.</searchLink><relatesTo>2</relatesTo><i> Donald.M.Cropek@usace.army.mil</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Yingxiao%22">Wang, Yingxiao</searchLink><relatesTo>1,4,5,6</relatesTo><i> yiw015@eng.ucsd.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomaterials%22">Biomaterials</searchLink>. May2013, Vol. 34 Issue 15, p3807-3815. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Platelet-derived+growth+factor%22">Platelet-derived growth factor</searchLink><br /><searchLink fieldCode="DE" term="%22Integrins%22">Integrins</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Focal+adhesions%22">Focal adhesions</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+migration%22">Cell migration</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+resonance+energy+transfer%22">Fluorescence resonance energy transfer</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Reactive oxygen species (ROS) have been shown to play crucial roles in regulating various cellular functions, e.g. focal adhesion (FA) dynamics and cell migration upon growth factor stimulation. However, it is not clear how ROS are regulated at subcellular FA sites to impact cell migration. We have developed a biosensor capable of monitoring ROS production at FA sites in live cells with high sensitivity and specificity, utilizing fluorescence resonance energy transfer (FRET). The results revealed that platelet derived growth factor (PDGF) can induce ROS production at FA sites, which is mediated by Rac1 activation. In contrast, integrins, specifically integrin αvβ3, inhibits this local ROS production. The RhoA activity can mediate this inhibitory role of integrins in regulating ROS production. Therefore, PDGF and integrin αvβ3 coordinate to have an antagonistic effect in the ROS production at FA sites to regulate cell adhesion and migration. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.biomaterials.2013.01.092
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        Text: English
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        PageCount: 9
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      – SubjectFull: Platelet-derived growth factor
        Type: general
      – SubjectFull: Integrins
        Type: general
      – SubjectFull: Reactive oxygen species
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      – SubjectFull: Focal adhesions
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      – SubjectFull: Cell migration
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      – SubjectFull: Fluorescence resonance energy transfer
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      – TitleFull: The antagonistic roles of PDGF and integrin αvβ3 in regulating ROS production at focal adhesions
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            NameFull: Lin, Li-Jung
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              Text: May2013
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