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Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps.

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Title: Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps.
Authors: Jaekel, Ulrike1, Musat, Niculina2, Adam, Birgit2, Kuypers, Marcel2, Grundmann, Olav1, Musat, Florin1
Source: ISME Journal: Multidisciplinary Journal of Microbial Ecology. May2013, Vol. 7 Issue 5, p885-895. 11p.
Subjects: Anaerobic bacteria, Biodegradation, Propane, Butane, Marine pollution, Gas seepage, Hydrocarbons
Abstract: The short-chain, non-methane hydrocarbons propane and butane can contribute significantly to the carbon and sulfur cycles in marine environments affected by oil or natural gas seepage. In the present study, we enriched and identified novel propane and butane-degrading sulfate reducers from marine oil and gas cold seeps in the Gulf of Mexico and Hydrate Ridge. The enrichment cultures obtained were able to degrade simultaneously propane and butane, but not other gaseous alkanes. They were cold-adapted, showing highest sulfate-reduction rates between 16 and 20 °C. Analysis of 16S rRNA gene libraries, followed by whole-cell hybridizations with sequence-specific oligonucleotide probes showed that each enrichment culture was dominated by a unique phylotype affiliated with the Desulfosarcina-Desulfococcus cluster within the Deltaproteobacteria. These phylotypes formed a distinct phylogenetic cluster of propane and butane degraders, including sequences from environments associated with hydrocarbon seeps. Incubations with 13C-labeled substrates, hybridizations with sequence-specific probes and nanoSIMS analyses showed that cells of the dominant phylotypes were the first to become enriched in 13C, demonstrating that they were directly involved in hydrocarbon degradation. Furthermore, using the nanoSIMS data, carbon assimilation rates were calculated for the dominant cells in each enrichment culture. [ABSTRACT FROM AUTHOR]
Copyright of ISME Journal: Multidisciplinary Journal of Microbial Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 87106078
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PubTypeId: academicJournal
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  Data: Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps.
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  Data: <searchLink fieldCode="AR" term="%22Jaekel%2C+Ulrike%22">Jaekel, Ulrike</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Musat%2C+Niculina%22">Musat, Niculina</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Adam%2C+Birgit%22">Adam, Birgit</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kuypers%2C+Marcel%22">Kuypers, Marcel</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Grundmann%2C+Olav%22">Grundmann, Olav</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Musat%2C+Florin%22">Musat, Florin</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22ISME+Journal%3A+Multidisciplinary+Journal+of+Microbial+Ecology%22">ISME Journal: Multidisciplinary Journal of Microbial Ecology</searchLink>. May2013, Vol. 7 Issue 5, p885-895. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Anaerobic+bacteria%22">Anaerobic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Biodegradation%22">Biodegradation</searchLink><br /><searchLink fieldCode="DE" term="%22Propane%22">Propane</searchLink><br /><searchLink fieldCode="DE" term="%22Butane%22">Butane</searchLink><br /><searchLink fieldCode="DE" term="%22Marine+pollution%22">Marine pollution</searchLink><br /><searchLink fieldCode="DE" term="%22Gas+seepage%22">Gas seepage</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrocarbons%22">Hydrocarbons</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: The short-chain, non-methane hydrocarbons propane and butane can contribute significantly to the carbon and sulfur cycles in marine environments affected by oil or natural gas seepage. In the present study, we enriched and identified novel propane and butane-degrading sulfate reducers from marine oil and gas cold seeps in the Gulf of Mexico and Hydrate Ridge. The enrichment cultures obtained were able to degrade simultaneously propane and butane, but not other gaseous alkanes. They were cold-adapted, showing highest sulfate-reduction rates between 16 and 20 °C. Analysis of 16S rRNA gene libraries, followed by whole-cell hybridizations with sequence-specific oligonucleotide probes showed that each enrichment culture was dominated by a unique phylotype affiliated with the Desulfosarcina-Desulfococcus cluster within the Deltaproteobacteria. These phylotypes formed a distinct phylogenetic cluster of propane and butane degraders, including sequences from environments associated with hydrocarbon seeps. Incubations with 13C-labeled substrates, hybridizations with sequence-specific probes and nanoSIMS analyses showed that cells of the dominant phylotypes were the first to become enriched in 13C, demonstrating that they were directly involved in hydrocarbon degradation. Furthermore, using the nanoSIMS data, carbon assimilation rates were calculated for the dominant cells in each enrichment culture. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of ISME Journal: Multidisciplinary Journal of Microbial Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1038/ismej.2012.159
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      – Code: eng
        Text: English
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        PageCount: 11
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      – SubjectFull: Anaerobic bacteria
        Type: general
      – SubjectFull: Biodegradation
        Type: general
      – SubjectFull: Propane
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      – SubjectFull: Butane
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      – SubjectFull: Marine pollution
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      – SubjectFull: Gas seepage
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      – SubjectFull: Hydrocarbons
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      – TitleFull: Anaerobic degradation of propane and butane by sulfate-reducing bacteria enriched from marine hydrocarbon cold seeps.
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            NameFull: Musat, Niculina
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              Text: May2013
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