Metabolic engineering of Candida utilis for isopropanol production.

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Bibliographic Details
Title:	Metabolic engineering of Candida utilis for isopropanol production.
Authors:	Tamakawa, Hideyuki¹ Hideyuki_Tamakawa@kirin.co.jp, Mita, Tokiko¹, Yokoyama, Aki¹, Ikushima, Shigehito¹, Yoshida, Satoshi¹
Source:	Applied Microbiology & Biotechnology. Jul2013, Vol. 97 Issue 14, p6231-6239. 9p.
Subjects:	Candida utilis, Genetic code, Isopropyl alcohol, Clostridium beijerinckii, Alcohol dehydrogenase, Acetyltransferases
Abstract:	A genetically-engineered strain of the yeast Candida utilis harboring genes encoding (1) an acetoacetyl-CoA transferase from Clostridium acetobutylicum ATCC 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from Clostridium beijerinckii NRRL B593 produced up to 0.21 g/L of isopropanol. Because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/L under neutralized fermentation conditions. Optimization of isopropanol production was attempted by the overexpression and disruption of several endogenous genes. Simultaneous overexpression of two genes encoding acetyl-CoA synthetase and acetyl-CoA acetyltransferase increased isopropanol titer to 9.5 g/L. Moreover, in fed-batch cultivation, the resultant recombinant strain produced 27.2 g/L of isopropanol from glucose with a yield of 41.5 % (mol/mol). This is the first demonstration of the production of isopropanol by genetically engineered yeast. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	A genetically-engineered strain of the yeast Candida utilis harboring genes encoding (1) an acetoacetyl-CoA transferase from Clostridium acetobutylicum ATCC 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from Clostridium beijerinckii NRRL B593 produced up to 0.21 g/L of isopropanol. Because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/L under neutralized fermentation conditions. Optimization of isopropanol production was attempted by the overexpression and disruption of several endogenous genes. Simultaneous overexpression of two genes encoding acetyl-CoA synthetase and acetyl-CoA acetyltransferase increased isopropanol titer to 9.5 g/L. Moreover, in fed-batch cultivation, the resultant recombinant strain produced 27.2 g/L of isopropanol from glucose with a yield of 41.5 % (mol/mol). This is the first demonstration of the production of isopropanol by genetically engineered yeast. [ABSTRACT FROM AUTHOR]
ISSN:	01757598
DOI:	10.1007/s00253-013-4964-0