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Metabolic engineering of Candida utilis for isopropanol production.

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Title: Metabolic engineering of Candida utilis for isopropanol production.
Authors: Tamakawa, Hideyuki1 Hideyuki_Tamakawa@kirin.co.jp, Mita, Tokiko1, Yokoyama, Aki1, Ikushima, Shigehito1, Yoshida, Satoshi1
Source: Applied Microbiology & Biotechnology. Jul2013, Vol. 97 Issue 14, p6231-6239. 9p.
Subjects: Candida utilis, Genetic code, Isopropyl alcohol, Clostridium beijerinckii, Alcohol dehydrogenase, Acetyltransferases
Abstract: A genetically-engineered strain of the yeast Candida utilis harboring genes encoding (1) an acetoacetyl-CoA transferase from Clostridium acetobutylicum ATCC 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from Clostridium beijerinckii NRRL B593 produced up to 0.21 g/L of isopropanol. Because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/L under neutralized fermentation conditions. Optimization of isopropanol production was attempted by the overexpression and disruption of several endogenous genes. Simultaneous overexpression of two genes encoding acetyl-CoA synthetase and acetyl-CoA acetyltransferase increased isopropanol titer to 9.5 g/L. Moreover, in fed-batch cultivation, the resultant recombinant strain produced 27.2 g/L of isopropanol from glucose with a yield of 41.5 % (mol/mol). This is the first demonstration of the production of isopropanol by genetically engineered yeast. [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Metabolic engineering of Candida utilis for isopropanol production.
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  Data: <searchLink fieldCode="AR" term="%22Tamakawa%2C+Hideyuki%22">Tamakawa, Hideyuki</searchLink><relatesTo>1</relatesTo><i> Hideyuki_Tamakawa@kirin.co.jp</i><br /><searchLink fieldCode="AR" term="%22Mita%2C+Tokiko%22">Mita, Tokiko</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yokoyama%2C+Aki%22">Yokoyama, Aki</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ikushima%2C+Shigehito%22">Ikushima, Shigehito</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yoshida%2C+Satoshi%22">Yoshida, Satoshi</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Jul2013, Vol. 97 Issue 14, p6231-6239. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Candida+utilis%22">Candida utilis</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+code%22">Genetic code</searchLink><br /><searchLink fieldCode="DE" term="%22Isopropyl+alcohol%22">Isopropyl alcohol</searchLink><br /><searchLink fieldCode="DE" term="%22Clostridium+beijerinckii%22">Clostridium beijerinckii</searchLink><br /><searchLink fieldCode="DE" term="%22Alcohol+dehydrogenase%22">Alcohol dehydrogenase</searchLink><br /><searchLink fieldCode="DE" term="%22Acetyltransferases%22">Acetyltransferases</searchLink>
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  Data: A genetically-engineered strain of the yeast Candida utilis harboring genes encoding (1) an acetoacetyl-CoA transferase from Clostridium acetobutylicum ATCC 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from Clostridium beijerinckii NRRL B593 produced up to 0.21 g/L of isopropanol. Because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/L under neutralized fermentation conditions. Optimization of isopropanol production was attempted by the overexpression and disruption of several endogenous genes. Simultaneous overexpression of two genes encoding acetyl-CoA synthetase and acetyl-CoA acetyltransferase increased isopropanol titer to 9.5 g/L. Moreover, in fed-batch cultivation, the resultant recombinant strain produced 27.2 g/L of isopropanol from glucose with a yield of 41.5 % (mol/mol). This is the first demonstration of the production of isopropanol by genetically engineered yeast. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00253-013-4964-0
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        Text: English
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        Type: general
      – SubjectFull: Genetic code
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      – SubjectFull: Isopropyl alcohol
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      – SubjectFull: Clostridium beijerinckii
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      – SubjectFull: Acetyltransferases
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              Text: Jul2013
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