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Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1.

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Title: Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1.
Authors: Sim, L, Ward, O P, Li, Z-Y
Source: Journal of Industrial Microbiology & Biotechnology. Oct97, Vol. 19 Issue 4, p232. 7p.
Subjects: Pseudomonas aeruginosa, Biosurfactants
Abstract: Pseudomonas aeruginosa UW-1 produced 17-24 g L[SUP-1] rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days. In time course studies of growth and rhamnolipid production in a salts medium containing 6% canola oil, total bacterial count reached 2.6 x 10[SUP10] CFU ml[SUP-1] after 48 h and a maximum rhamnolipid yield of 24.3 g L[SUP-1] was obtained after 9 days. Rhamnolipid components were purified and separated by chloroform-methanol extraction and TLC chromatography. The major rhamnolipid components were characterised as L-rhamnosyl-β-hydroxy-decanoyl-β-hydroxydecanoate and L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate by nuclear magnetic resonance and mass spectrometry. The components were separated preparatively by silica gel column chromatography. The recovered monorhamnosyl fraction contained no dirhamnosyl moiety while the recovered dirhamnosyl fraction contained 5% of the monorhamnosyl moiety when analyzed by HPLC. The ratio of mono- to dirhamnosyl components produced by P. aeruginosa UW-1 was determined by HPLC to be 4 : 1 by weight. Purified mono- and dirhamnosyl components had the same CMC value of 40 μg ml[SUP-1] and decreased the surface tension of water to 27.7 and 30.4 dynes cm[SUP-1], respectively. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1.
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Industrial+Microbiology+%26+Biotechnology%22">Journal of Industrial Microbiology & Biotechnology</searchLink>. Oct97, Vol. 19 Issue 4, p232. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Pseudomonas+aeruginosa%22">Pseudomonas aeruginosa</searchLink><br /><searchLink fieldCode="DE" term="%22Biosurfactants%22">Biosurfactants</searchLink>
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  Data: Pseudomonas aeruginosa UW-1 produced 17-24 g L[SUP-1] rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days. In time course studies of growth and rhamnolipid production in a salts medium containing 6% canola oil, total bacterial count reached 2.6 x 10[SUP10] CFU ml[SUP-1] after 48 h and a maximum rhamnolipid yield of 24.3 g L[SUP-1] was obtained after 9 days. Rhamnolipid components were purified and separated by chloroform-methanol extraction and TLC chromatography. The major rhamnolipid components were characterised as L-rhamnosyl-β-hydroxy-decanoyl-β-hydroxydecanoate and L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate by nuclear magnetic resonance and mass spectrometry. The components were separated preparatively by silica gel column chromatography. The recovered monorhamnosyl fraction contained no dirhamnosyl moiety while the recovered dirhamnosyl fraction contained 5% of the monorhamnosyl moiety when analyzed by HPLC. The ratio of mono- to dirhamnosyl components produced by P. aeruginosa UW-1 was determined by HPLC to be 4 : 1 by weight. Purified mono- and dirhamnosyl components had the same CMC value of 40 μg ml[SUP-1] and decreased the surface tension of water to 27.7 and 30.4 dynes cm[SUP-1], respectively. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1038/sj.jim.2900450
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      – Code: eng
        Text: English
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      – SubjectFull: Biosurfactants
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      – TitleFull: Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1.
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              Text: Oct97
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              Y: 1997
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