Bibliographic Details
| Title: |
Bead assembly magnetorotation as a signal transduction method for protein detection. |
| Authors: |
Hecht, Ariel1,2, Commiskey, Patrick2,3, Shah, Nicholas2,4, Kopelman, Raoul1,2,3 kopelman@umich.edu |
| Source: |
Biosensors & Bioelectronics. Oct2013, Vol. 48, p26-32. 7p. |
| Subjects: |
Cellular signal transduction, Aptamers, Thrombin, Magnetic materials, Fractal dimensions, Lasers |
| Abstract: |
Abstract: This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1μm magnetic beads into a bead assembly, formed at the bottom of a 1μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field. [Copyright &y& Elsevier] |
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| Database: |
Engineering Source |
