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Bead assembly magnetorotation as a signal transduction method for protein detection.

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Title: Bead assembly magnetorotation as a signal transduction method for protein detection.
Authors: Hecht, Ariel1,2, Commiskey, Patrick2,3, Shah, Nicholas2,4, Kopelman, Raoul1,2,3 kopelman@umich.edu
Source: Biosensors & Bioelectronics. Oct2013, Vol. 48, p26-32. 7p.
Subjects: Cellular signal transduction, Aptamers, Thrombin, Magnetic materials, Fractal dimensions, Lasers
Abstract: Abstract: This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1μm magnetic beads into a bead assembly, formed at the bottom of a 1μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field. [Copyright &y& Elsevier]
Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 89278987
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Bead assembly magnetorotation as a signal transduction method for protein detection.
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  Data: <searchLink fieldCode="AR" term="%22Hecht%2C+Ariel%22">Hecht, Ariel</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Commiskey%2C+Patrick%22">Commiskey, Patrick</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Shah%2C+Nicholas%22">Shah, Nicholas</searchLink><relatesTo>2,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Kopelman%2C+Raoul%22">Kopelman, Raoul</searchLink><relatesTo>1,2,3</relatesTo><i> kopelman@umich.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Biosensors+%26+Bioelectronics%22">Biosensors & Bioelectronics</searchLink>. Oct2013, Vol. 48, p26-32. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Aptamers%22">Aptamers</searchLink><br /><searchLink fieldCode="DE" term="%22Thrombin%22">Thrombin</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+materials%22">Magnetic materials</searchLink><br /><searchLink fieldCode="DE" term="%22Fractal+dimensions%22">Fractal dimensions</searchLink><br /><searchLink fieldCode="DE" term="%22Lasers%22">Lasers</searchLink>
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  Data: Abstract: This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1μm magnetic beads into a bead assembly, formed at the bottom of a 1μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.bios.2013.03.073
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 26
    Subjects:
      – SubjectFull: Cellular signal transduction
        Type: general
      – SubjectFull: Aptamers
        Type: general
      – SubjectFull: Thrombin
        Type: general
      – SubjectFull: Magnetic materials
        Type: general
      – SubjectFull: Fractal dimensions
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      – SubjectFull: Lasers
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      – TitleFull: Bead assembly magnetorotation as a signal transduction method for protein detection.
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            NameFull: Shah, Nicholas
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              Text: Oct2013
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              Y: 2013
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              Value: 48
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