In Vitro Effects of Isoorientin, Forsythoside B, and Verbascoside on Bovine Kidney Cortex Glutathione Reductase.
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| Title: | In Vitro Effects of Isoorientin, Forsythoside B, and Verbascoside on Bovine Kidney Cortex Glutathione Reductase. |
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| Authors: | Tandogan, Berivan1, Guvenc, Ayşegül2, Calis, Ihsan3, Ulusu, Nuriye Nuray1 |
| Source: | International Journal of Chemical Kinetics. Sep2013, Vol. 45 Issue 9, p574-579. 6p. |
| Subjects: | Orienting reflex, Verbascoside, Glutathione reductase, Free radicals, Plant species, Reactive oxygen species, Drug resistance, Catalysts |
| Abstract: | ABSTRACT Glutathione reductase (GR; E.C 1.6.4.2) catalyzes the reduction of oxidized glutathione to reduced glutathione by the nicotinamide adenine dinucleotide phosphate (NADPH) dependent mechanism. GR participates in the protection of the cell from the toxic effects of free radicals. In this study, we have investigated in vitro effects of isoorientin ( C-glycosyl flavonoid), verbascoside, and forsythoside B (phenylethanoids) on purified bovine kidney cortex GR. These compounds present in many plant species that have been used as medicinal plants and have an interesting spectrum of biological activity. We have demonstrated that these compounds inhibit bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition is also done. Isoorientin is a noncompetitive inhibitor with respect to both glutathione disulfide (GSSG) ( KiGSSG 0.023 ± 0.001 mM) and NADPH ( KiNADPH 0.032 ± 0.001 mM). Forsythoside B acts as an uncompetitive inhibitor with respect to GSSG ( KiGSSG 0.20 ± 0.014 mM) and NADPH ( KiNADPH 0.267 ± 0.028 mM). Verbascoside acts as an uncompetitive inhibitor with respect to both GSSG ( KiGSSG 0.242 ± 0.024 mM) and NADPH ( KiNADPH 0.175 ± 0.016 mM). Inhibition of GR causes accumulation of reactive oxygen species and depletion of the glutathione pool. Inhibition of this enzyme may be significant in drug resistance. [ABSTRACT FROM AUTHOR] |
| Copyright of International Journal of Chemical Kinetics is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 89305589 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: In Vitro Effects of Isoorientin, Forsythoside B, and Verbascoside on Bovine Kidney Cortex Glutathione Reductase. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tandogan%2C+Berivan%22">Tandogan, Berivan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Guvenc%2C+Ayşegül%22">Guvenc, Ayşegül</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Calis%2C+Ihsan%22">Calis, Ihsan</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ulusu%2C+Nuriye+Nuray%22">Ulusu, Nuriye Nuray</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22International+Journal+of+Chemical+Kinetics%22">International Journal of Chemical Kinetics</searchLink>. Sep2013, Vol. 45 Issue 9, p574-579. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Orienting+reflex%22">Orienting reflex</searchLink><br /><searchLink fieldCode="DE" term="%22Verbascoside%22">Verbascoside</searchLink><br /><searchLink fieldCode="DE" term="%22Glutathione+reductase%22">Glutathione reductase</searchLink><br /><searchLink fieldCode="DE" term="%22Free+radicals%22">Free radicals</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+species%22">Plant species</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+resistance%22">Drug resistance</searchLink><br /><searchLink fieldCode="DE" term="%22Catalysts%22">Catalysts</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: ABSTRACT Glutathione reductase (GR; E.C 1.6.4.2) catalyzes the reduction of oxidized glutathione to reduced glutathione by the nicotinamide adenine dinucleotide phosphate (NADPH) dependent mechanism. GR participates in the protection of the cell from the toxic effects of free radicals. In this study, we have investigated in vitro effects of isoorientin ( C-glycosyl flavonoid), verbascoside, and forsythoside B (phenylethanoids) on purified bovine kidney cortex GR. These compounds present in many plant species that have been used as medicinal plants and have an interesting spectrum of biological activity. We have demonstrated that these compounds inhibit bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition is also done. Isoorientin is a noncompetitive inhibitor with respect to both glutathione disulfide (GSSG) ( KiGSSG 0.023 ± 0.001 mM) and NADPH ( KiNADPH 0.032 ± 0.001 mM). Forsythoside B acts as an uncompetitive inhibitor with respect to GSSG ( KiGSSG 0.20 ± 0.014 mM) and NADPH ( KiNADPH 0.267 ± 0.028 mM). Verbascoside acts as an uncompetitive inhibitor with respect to both GSSG ( KiGSSG 0.242 ± 0.024 mM) and NADPH ( KiNADPH 0.175 ± 0.016 mM). Inhibition of GR causes accumulation of reactive oxygen species and depletion of the glutathione pool. Inhibition of this enzyme may be significant in drug resistance. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of International Journal of Chemical Kinetics is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/kin.20792 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 574 Subjects: – SubjectFull: Orienting reflex Type: general – SubjectFull: Verbascoside Type: general – SubjectFull: Glutathione reductase Type: general – SubjectFull: Free radicals Type: general – SubjectFull: Plant species Type: general – SubjectFull: Reactive oxygen species Type: general – SubjectFull: Drug resistance Type: general – SubjectFull: Catalysts Type: general Titles: – TitleFull: In Vitro Effects of Isoorientin, Forsythoside B, and Verbascoside on Bovine Kidney Cortex Glutathione Reductase. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tandogan, Berivan – PersonEntity: Name: NameFull: Guvenc, Ayşegül – PersonEntity: Name: NameFull: Calis, Ihsan – PersonEntity: Name: NameFull: Ulusu, Nuriye Nuray IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 05388066 Numbering: – Type: volume Value: 45 – Type: issue Value: 9 Titles: – TitleFull: International Journal of Chemical Kinetics Type: main |
| ResultId | 1 |
