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Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat.

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Title: Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat.
Authors: Bassi, F. filippo.bassi@ndsu.edu, Kumar, A.1, Zhang, Q.1, Paux, E.2, Huttner, E.3 Eric.Huttner@aciar.gov.au, Kilian, A.4, Dizon, R.1, Feuillet, C.2, Xu, S., Kianian, S.1 S.Kianian@ndsu.edu
Source: Theoretical & Applied Genetics. Aug2013, Vol. 126 Issue 8, p1977-1990. 14p.
Subjects: Wheat, Vegetation mapping, Plant cytogenetics, Plant genes, Plant chromosomes, Sterility in plants, Plant clones
Abstract: Since the dawn of wheat cytogenetics, chromosome 3B has been known to harbor a gene(s) that, when removed, causes chromosome desynapsis and gametic sterility. The lack of natural genetic diversity for this gene(s) has prevented any attempt to fine map and further characterize it. Here, gamma radiation treatment was used to create artificial diversity for this locus. A total of 696 radiation hybrid lines were genotyped with a custom mini array of 140 DArT markers, selected to evenly span the whole 3B chromosome. The resulting map spanned 2,852 centi Ray with a calculated resolution of 0.384 Mb. Phenotyping for the occurrence of meiotic desynapsis was conducted by measuring the level of gametic sterility as seeds produced per spikelet and pollen viability at booting. Composite interval mapping revealed a single QTL with LOD of 16.2 and r of 25.6 % between markers wmc326 and wPt- 8983 on the long arm of chromosome 3B. By independent analysis, the location of the QTL was confirmed to be within the deletion bin 3BL7-0.63-1.00 and to correspond to a single gene located ~1.4 Mb away from wPt- 8983. The meiotic behavior of lines lacking this gene was characterized cytogenetically to reveal striking similarities with mutants for the dy locus, located on the syntenic chromosome 3 of maize. This represents the first example to date of employing radiation hybrids for QTL analysis. The success achieved by this approach provides an ideal starting point for the final cloning of this interesting gene involved in meiosis of cereals. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat.
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  Data: <searchLink fieldCode="AR" term="%22Bassi%2C+F%2E%22">Bassi, F.</searchLink><i> filippo.bassi@ndsu.edu</i><br /><searchLink fieldCode="AR" term="%22Kumar%2C+A%2E%22">Kumar, A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Q%2E%22">Zhang, Q.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Paux%2C+E%2E%22">Paux, E.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Huttner%2C+E%2E%22">Huttner, E.</searchLink><relatesTo>3</relatesTo><i> Eric.Huttner@aciar.gov.au</i><br /><searchLink fieldCode="AR" term="%22Kilian%2C+A%2E%22">Kilian, A.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Dizon%2C+R%2E%22">Dizon, R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Feuillet%2C+C%2E%22">Feuillet, C.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xu%2C+S%2E%22">Xu, S.</searchLink><br /><searchLink fieldCode="AR" term="%22Kianian%2C+S%2E%22">Kianian, S.</searchLink><relatesTo>1</relatesTo><i> S.Kianian@ndsu.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Aug2013, Vol. 126 Issue 8, p1977-1990. 14p.
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  Data: <searchLink fieldCode="DE" term="%22Wheat%22">Wheat</searchLink><br /><searchLink fieldCode="DE" term="%22Vegetation+mapping%22">Vegetation mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+cytogenetics%22">Plant cytogenetics</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genes%22">Plant genes</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+chromosomes%22">Plant chromosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Sterility+in+plants%22">Sterility in plants</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+clones%22">Plant clones</searchLink>
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  Data: Since the dawn of wheat cytogenetics, chromosome 3B has been known to harbor a gene(s) that, when removed, causes chromosome desynapsis and gametic sterility. The lack of natural genetic diversity for this gene(s) has prevented any attempt to fine map and further characterize it. Here, gamma radiation treatment was used to create artificial diversity for this locus. A total of 696 radiation hybrid lines were genotyped with a custom mini array of 140 DArT markers, selected to evenly span the whole 3B chromosome. The resulting map spanned 2,852 centi Ray with a calculated resolution of 0.384 Mb. Phenotyping for the occurrence of meiotic desynapsis was conducted by measuring the level of gametic sterility as seeds produced per spikelet and pollen viability at booting. Composite interval mapping revealed a single QTL with LOD of 16.2 and r of 25.6 % between markers wmc326 and wPt- 8983 on the long arm of chromosome 3B. By independent analysis, the location of the QTL was confirmed to be within the deletion bin 3BL7-0.63-1.00 and to correspond to a single gene located ~1.4 Mb away from wPt- 8983. The meiotic behavior of lines lacking this gene was characterized cytogenetically to reveal striking similarities with mutants for the dy locus, located on the syntenic chromosome 3 of maize. This represents the first example to date of employing radiation hybrids for QTL analysis. The success achieved by this approach provides an ideal starting point for the final cloning of this interesting gene involved in meiosis of cereals. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        StartPage: 1977
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      – SubjectFull: Wheat
        Type: general
      – SubjectFull: Vegetation mapping
        Type: general
      – SubjectFull: Plant cytogenetics
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      – SubjectFull: Sterility in plants
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