Accurate Method for the Determination of Intramolecular 13C Isotope Composition of Ethanol from Aqueous Solutions.
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| Title: | Accurate Method for the Determination of Intramolecular 13C Isotope Composition of Ethanol from Aqueous Solutions. |
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| Authors: | Gilbert, Alexis1 gilbert.a.aa@m.titech.ac.jp, Yamada, Keita1, Yoshida, Naohiro1,2 |
| Source: | Analytical Chemistry. 7/16/2013, Vol. 85 Issue 14, p6566-6570. 5p. |
| Subjects: | Carbon isotopes, Ethanol, Solid phase extraction, Pyrolysis, Mass spectrometry, Intramolecular isotope effects, Alcoholic beverage analysis |
| Abstract: | A new method, combining headspace solid phase microextraction (HS-SPME) with an online pyrolysis system coupled with isotope ratio mass spectrometry (IRMS), is developed for the determination of the intramolecular 13C isotope composition of ethanol in aqueous solutions. The δ13C values of the pyrolytic fragments (CO, CH4, C2H4) are shown to be highly reproducible (sd <0.4‰). Furthermore, using 14 ethanol samples of known intramolecular isotope distribution, the CO and CH4 fragments are shown to arise solely from the methylene (CH2OH) and methyl (CH3) carbon atom positions of the original ethanol, respectively. Although the different steps (extraction and pyrolysis) fractionate between 12C and 13C, the isotopic fractionation is reproducible (sd <0.4‰), allowing correcting factors to be applied in order to back-calculate the original δ13CCH2OH and δ13CCH3 values of ethanol. The method thus allows the determination of the isotope composition of ethanol at the intramolecular and molecular levels, within a single run and a short experimental time (30 min), and with a very easy sample preparation. The method is then applied to alcoholic beverages to show its potential for authentication purposes. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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