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A REVERSED-PHASE HPLC METHOD DEVELOPMENT FOR THE SEPARATION OF NEW ANTIDEPRESSANTS.

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Title: A REVERSED-PHASE HPLC METHOD DEVELOPMENT FOR THE SEPARATION OF NEW ANTIDEPRESSANTS.
Authors: Dallet, P.1 (AUTHOR) philippe.dallet@u-bordeaux2.fr, Labat, L.1 (AUTHOR), Richard, M.1 (AUTHOR), Langlois, M.H.1 (AUTHOR), Dubost, J.P.1 (AUTHOR)
Source: Journal of Liquid Chromatography & Related Technologies. 1/1/2002, Vol. 25 Issue 1, p101-111. 11p.
Subjects: High performance liquid chromatography, Antidepressants
Abstract: A RPLC method with UV detection (225 nm) is developed for the separation of five SSRIs (fluvoxamine, fluoxetine, sertraline, paroxetine, and citalopram), two SNaRIS (venlafaxine and milnacipran), one NaSSA (mirtazapine), and four active metabolites (norfluoxetine, desmethylcitalopram, desmethylvenlafaxine, and desmethylmirtazapine). A standard solution (20 μg/mL) of the twelve compounds is analysed under isocratic conditions on two new-generation RP columns (Satisfaction® RP 18 AB and Satisfaction® C8+, 250 mm × 4.6 mm, 5 μm). Mobile phase composition (acetonitrile content, pH of the aqueous buffer) and temperature are varied and the effect of these parameters on the retention factors of the antidepressants is examined. Similar elution profiles are observed with the two stationary phases, but the separation of all the solutes is only possible on the RP 18 AB column. It can be achieved at 45°C (or 50°C) with a mobile phase consisting of a mixture of potassium dihydrogen phosphate (pH 4.8, 25 mM)-acetonitrile (65:35, v/v) (flow rate: 1 mL/min). The run time is 20 min and a baseline resolution is obtained for all the analytes allowing this procedure to be well suited for a rapid toxicological screening. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A REVERSED-PHASE HPLC METHOD DEVELOPMENT FOR THE SEPARATION OF NEW ANTIDEPRESSANTS.
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Liquid+Chromatography+%26+Related+Technologies%22">Journal of Liquid Chromatography & Related Technologies</searchLink>. 1/1/2002, Vol. 25 Issue 1, p101-111. 11p.
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– Name: Abstract
  Label: Abstract
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  Data: A RPLC method with UV detection (225 nm) is developed for the separation of five SSRIs (fluvoxamine, fluoxetine, sertraline, paroxetine, and citalopram), two SNaRIS (venlafaxine and milnacipran), one NaSSA (mirtazapine), and four active metabolites (norfluoxetine, desmethylcitalopram, desmethylvenlafaxine, and desmethylmirtazapine). A standard solution (20 μg/mL) of the twelve compounds is analysed under isocratic conditions on two new-generation RP columns (Satisfaction® RP 18 AB and Satisfaction® C8+, 250 mm × 4.6 mm, 5 μm). Mobile phase composition (acetonitrile content, pH of the aqueous buffer) and temperature are varied and the effect of these parameters on the retention factors of the antidepressants is examined. Similar elution profiles are observed with the two stationary phases, but the separation of all the solutes is only possible on the RP 18 AB column. It can be achieved at 45°C (or 50°C) with a mobile phase consisting of a mixture of potassium dihydrogen phosphate (pH 4.8, 25 mM)-acetonitrile (65:35, v/v) (flow rate: 1 mL/min). The run time is 20 min and a baseline resolution is obtained for all the analytes allowing this procedure to be well suited for a rapid toxicological screening. [ABSTRACT FROM AUTHOR]
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  Group: Ab
  Data: <i>Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1081/JLC-100108542
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              Text: 1/1/2002
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