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Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli.

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Title: Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli.
Authors: Fayura, Lyubov R.1, Boretsky, Yuriy R.1, Pynyaha, Yuriy V.1, Wheatley, Denys N.2, Sibirny, Andriy A.1,3 sibirny@cellbiol.lviv.ua
Source: Journal of Biotechnology. Sep2013, Vol. 167 Issue 4, p420-426. 7p.
Subjects: Mycoplasma, Arginine deiminase, Gene expression, Chemical reagents, Bacterial genetics, Cost effectiveness, Escherichia coli
Abstract: Highlights: [•] M. hominis gene coding for arginine deiminase was cloned and expressed successfully. [•] The recombinant arginine deiminase producer is stabilized significantly. [•] The expensive reagents are substituted with cheaper ones. [•] The expressed protein has been purified successfully. [•] As a result low-cost method for high-efficient production of arginine deiminase is developed. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 90302521
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli.
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  Data: <searchLink fieldCode="AR" term="%22Fayura%2C+Lyubov+R%2E%22">Fayura, Lyubov R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Boretsky%2C+Yuriy+R%2E%22">Boretsky, Yuriy R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pynyaha%2C+Yuriy+V%2E%22">Pynyaha, Yuriy V.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wheatley%2C+Denys+N%2E%22">Wheatley, Denys N.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sibirny%2C+Andriy+A%2E%22">Sibirny, Andriy A.</searchLink><relatesTo>1,3</relatesTo><i> sibirny@cellbiol.lviv.ua</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biotechnology%22">Journal of Biotechnology</searchLink>. Sep2013, Vol. 167 Issue 4, p420-426. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Mycoplasma%22">Mycoplasma</searchLink><br /><searchLink fieldCode="DE" term="%22Arginine+deiminase%22">Arginine deiminase</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+reagents%22">Chemical reagents</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+genetics%22">Bacterial genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Cost+effectiveness%22">Cost effectiveness</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Highlights: [•] M. hominis gene coding for arginine deiminase was cloned and expressed successfully. [•] The recombinant arginine deiminase producer is stabilized significantly. [•] The expensive reagents are substituted with cheaper ones. [•] The expressed protein has been purified successfully. [•] As a result low-cost method for high-efficient production of arginine deiminase is developed. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jbiotec.2013.06.025
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      – Code: eng
        Text: English
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        StartPage: 420
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      – SubjectFull: Mycoplasma
        Type: general
      – SubjectFull: Arginine deiminase
        Type: general
      – SubjectFull: Gene expression
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      – SubjectFull: Chemical reagents
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      – SubjectFull: Bacterial genetics
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      – SubjectFull: Cost effectiveness
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      – SubjectFull: Escherichia coli
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              Text: Sep2013
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