Comparison of standard capillary and chip separations of sodium dodecylsulfate–protein complexes
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| Title: | Comparison of standard capillary and chip separations of sodium dodecylsulfate–protein complexes |
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| Authors: | Deyl, Zden&ebreve;k deyl@biomed.cas.cz, Mikšık, Ivan1, Eckhardt, Adam1 |
| Source: | Journal of Chromatography A. Mar2003, Vol. 990 Issue 1/2, p153. 6p. |
| Subjects: | Capillary electrophoresis, Proteins |
| Abstract: | Conditions for converting a set of five standard proteins to electrochemically active sodium dodecylsulfate (SDS) complexes were worked out with the aim of using such complexes for conductivity detection with a a chip electrophoresis system. The results obtained were compared with standard capillary electrophoresis (37 cm (effective length 30 cm)×75 μm I.D. capillary, 10 kV, negative polarity at the inlet). The chip separations were run at 500 V per chip (100 V/cm) as compared to the standard capillary arrangement, which was run at 266.6 V/cm. For the capillary set-up the protein complexes were prepared in aqueous solution (Milli-Q water) made 10 mM with respect to SDS. If the SDS concentration was increased to 50 mM, the separation in the capillary was incomplete. On the other hand with the chip system both approaches yielded acceptable results. The chip separations were slightly (but not distinctly) shorter and offered better separations than the standard set-up. The concentration of the surfactant used for the preparation the complexes results in alternations of the elution sequence, which is preserved if the chip separation is used instead of the capillary set-up. Apparently the full capacity of protein–SDS binding is not exploited for the preparation of the adducts. [Copyright &y& Elsevier] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 9231451 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Comparison of standard capillary and chip separations of sodium dodecylsulfate–protein complexes – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Deyl%2C+Zden%26ebreve%3Bk%22">Deyl, Zden&ebreve;k</searchLink><i> deyl@biomed.cas.cz</i><br /><searchLink fieldCode="AR" term="%22Mikšık%2C+Ivan%22">Mikšık, Ivan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Eckhardt%2C+Adam%22">Eckhardt, Adam</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Mar2003, Vol. 990 Issue 1/2, p153. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Capillary+electrophoresis%22">Capillary electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Conditions for converting a set of five standard proteins to electrochemically active sodium dodecylsulfate (SDS) complexes were worked out with the aim of using such complexes for conductivity detection with a a chip electrophoresis system. The results obtained were compared with standard capillary electrophoresis (37 cm (effective length 30 cm)×75 μm I.D. capillary, 10 kV, negative polarity at the inlet). The chip separations were run at 500 V per chip (100 V/cm) as compared to the standard capillary arrangement, which was run at 266.6 V/cm. For the capillary set-up the protein complexes were prepared in aqueous solution (Milli-Q water) made 10 mM with respect to SDS. If the SDS concentration was increased to 50 mM, the separation in the capillary was incomplete. On the other hand with the chip system both approaches yielded acceptable results. The chip separations were slightly (but not distinctly) shorter and offered better separations than the standard set-up. The concentration of the surfactant used for the preparation the complexes results in alternations of the elution sequence, which is preserved if the chip separation is used instead of the capillary set-up. Apparently the full capacity of protein–SDS binding is not exploited for the preparation of the adducts. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/S0021-9673(02)01523-6 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 153 Subjects: – SubjectFull: Capillary electrophoresis Type: general – SubjectFull: Proteins Type: general Titles: – TitleFull: Comparison of standard capillary and chip separations of sodium dodecylsulfate–protein complexes Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Deyl, Zden&ebreve;k – PersonEntity: Name: NameFull: Mikšık, Ivan – PersonEntity: Name: NameFull: Eckhardt, Adam IsPartOfRelationships: – BibEntity: Dates: – D: 21 M: 03 Text: Mar2003 Type: published Y: 2003 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 990 – Type: issue Value: 1/2 Titles: – TitleFull: Journal of Chromatography A Type: main |
| ResultId | 1 |
