PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells.
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| Title: | PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells. |
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| Authors: | Kara, Altan1, Ünak, Perihan1 perihan.unak@gmail.com, Selçuki, Cenk2, Akça, Özlet1, Medine, E.1, Sakarya, Serhan3 |
| Source: | Journal of Radioanalytical & Nuclear Chemistry. Jan2014, Vol. 299 Issue 1, p807-813. 7p. |
| Subjects: | Poly-beta-hydroxyalkanoates, Cadmium selenide, Carbohydrates, Breast cancer, Bioconjugates, Cancer cells, Metal nanoparticles, Radiolabeling, Chemical affinity |
| Abstract: | This research aims to investigate the interaction between phytohemagglutinin-L (PHA-L) and sialic acid, which is abundant on the breast cancer cell (MCF-7) surface and displays monosaccharide characteristics, by experimental and computational methods. Experimentally, CdSe/CdS nanoparticles (QDs) were synthesized; PHA-L was conjugated with QDs and labeled with I. Radiolabeling yield was found to be 97 ± 1.2 %. Afterwards, in vitro bioaffinities of radiolabeled PHA-L conjugated QDs have been investigated on MCF-7 cells and it has been observed that the cell incorporation increased with time. The results indicated that I labeled QD-PHA-L conjugates represent significant affinity on MCF-7 cells. In the second step of the study, the crystal structure of carbohydrate interaction surface of PHA-L was extracted from the crystal structure of PHA-L. The interactions between this surface and sialic acid were calculated by computational tools. These calculations revealed specific interactions between PHA-L and sialic acid. Semi-empirical methods, PM3 and AM1, were used in these calculations. Significant outcomes have been obtained from the experimental and computational studies and these results demonstrated that PHA-L may be an effective agent for imagining MCF-7 cells. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Radioanalytical & Nuclear Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 93447166 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kara%2C+Altan%22">Kara, Altan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ünak%2C+Perihan%22">Ünak, Perihan</searchLink><relatesTo>1</relatesTo><i> perihan.unak@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Selçuki%2C+Cenk%22">Selçuki, Cenk</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Akça%2C+Özlet%22">Akça, Özlet</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Medine%2C+E%2E%22">Medine, E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sakarya%2C+Serhan%22">Sakarya, Serhan</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Radioanalytical+%26+Nuclear+Chemistry%22">Journal of Radioanalytical & Nuclear Chemistry</searchLink>. Jan2014, Vol. 299 Issue 1, p807-813. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Cadmium+selenide%22">Cadmium selenide</searchLink><br /><searchLink fieldCode="DE" term="%22Carbohydrates%22">Carbohydrates</searchLink><br /><searchLink fieldCode="DE" term="%22Breast+cancer%22">Breast cancer</searchLink><br /><searchLink fieldCode="DE" term="%22Bioconjugates%22">Bioconjugates</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+cells%22">Cancer cells</searchLink><br /><searchLink fieldCode="DE" term="%22Metal+nanoparticles%22">Metal nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Radiolabeling%22">Radiolabeling</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+affinity%22">Chemical affinity</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: This research aims to investigate the interaction between phytohemagglutinin-L (PHA-L) and sialic acid, which is abundant on the breast cancer cell (MCF-7) surface and displays monosaccharide characteristics, by experimental and computational methods. Experimentally, CdSe/CdS nanoparticles (QDs) were synthesized; PHA-L was conjugated with QDs and labeled with I. Radiolabeling yield was found to be 97 ± 1.2 %. Afterwards, in vitro bioaffinities of radiolabeled PHA-L conjugated QDs have been investigated on MCF-7 cells and it has been observed that the cell incorporation increased with time. The results indicated that I labeled QD-PHA-L conjugates represent significant affinity on MCF-7 cells. In the second step of the study, the crystal structure of carbohydrate interaction surface of PHA-L was extracted from the crystal structure of PHA-L. The interactions between this surface and sialic acid were calculated by computational tools. These calculations revealed specific interactions between PHA-L and sialic acid. Semi-empirical methods, PM3 and AM1, were used in these calculations. Significant outcomes have been obtained from the experimental and computational studies and these results demonstrated that PHA-L may be an effective agent for imagining MCF-7 cells. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Radioanalytical & Nuclear Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10967-013-2783-5 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 807 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Cadmium selenide Type: general – SubjectFull: Carbohydrates Type: general – SubjectFull: Breast cancer Type: general – SubjectFull: Bioconjugates Type: general – SubjectFull: Cancer cells Type: general – SubjectFull: Metal nanoparticles Type: general – SubjectFull: Radiolabeling Type: general – SubjectFull: Chemical affinity Type: general Titles: – TitleFull: PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kara, Altan – PersonEntity: Name: NameFull: Ünak, Perihan – PersonEntity: Name: NameFull: Selçuki, Cenk – PersonEntity: Name: NameFull: Akça, Özlet – PersonEntity: Name: NameFull: Medine, E. – PersonEntity: Name: NameFull: Sakarya, Serhan IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 02365731 Numbering: – Type: volume Value: 299 – Type: issue Value: 1 Titles: – TitleFull: Journal of Radioanalytical & Nuclear Chemistry Type: main |
| ResultId | 1 |
