Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm.
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| Title: | Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm. |
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| Authors: | Öğretmen, Fatih1 fatihogretmen@mu.edu.tr, İnanan, Burak E.2, Öztürk, Mehmet3 |
| Source: | Cryobiology. Feb2014, Vol. 68 Issue 1, p107-112. 6p. |
| Subjects: | Propolis, Cryopreservation of organs, tissues, etc., Carp, Artificial insemination, Sperm motility, Comparative studies |
| Abstract: | Abstract: Cryopreservation of sperm is common procedures in aquaculture, particularly used for routine in artificial insemination. However, these application cause damages and adversely affected sperm motility, viability and consequently lower hatching rates. The objective of this study is to determine whether propolis has an effect on cryopreservation and fertilization ability and to investigate the potential protective effect of propolis on spermatozoa of Cyprinus carpio. Many studies have been done in cryopreservation offish spermatozoa, but none of them contain propolis in extender composition. The extenders were prepared by using modified Kurokura Solution to which 10% Me2SO added with different levels of propolis (0.2, 0.4, 0.6, 0.8 and 1mgml−1) and 10% egg yolk (as a control without propolis). The pooled semen samples diluted at the ratio of 1:9 by the extenders were subjected to cryopreservation. The percentage and duration of motility and fertilization tests of cryopreserved sperm samples have been done immediately after thawing and compared with control and fresh semen. The extenders containing propolis exhibited higher percentage motility and motility duration than control group (P <0.05). Especially the group IV (0.8mgml−1 propolis) and the group V (1mgml−1 propolis) showed significant positive effects on both post thaw motility and hatching ability. The propolis maintained the integrity of the spermatozoa during the cryopreservation process. Evaluating with its contents, it has been shown that propolis is an appropriate cryoprotective agent in fish semen. [Copyright &y& Elsevier] |
| Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 94487528 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Öğretmen%2C+Fatih%22">Öğretmen, Fatih</searchLink><relatesTo>1</relatesTo><i> fatihogretmen@mu.edu.tr</i><br /><searchLink fieldCode="AR" term="%22İnanan%2C+Burak+E%2E%22">İnanan, Burak E.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Öztürk%2C+Mehmet%22">Öztürk, Mehmet</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Cryobiology%22">Cryobiology</searchLink>. Feb2014, Vol. 68 Issue 1, p107-112. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Propolis%22">Propolis</searchLink><br /><searchLink fieldCode="DE" term="%22Cryopreservation+of+organs%2C+tissues%2C+etc%2E%22">Cryopreservation of organs, tissues, etc.</searchLink><br /><searchLink fieldCode="DE" term="%22Carp%22">Carp</searchLink><br /><searchLink fieldCode="DE" term="%22Artificial+insemination%22">Artificial insemination</searchLink><br /><searchLink fieldCode="DE" term="%22Sperm+motility%22">Sperm motility</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+studies%22">Comparative studies</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: Cryopreservation of sperm is common procedures in aquaculture, particularly used for routine in artificial insemination. However, these application cause damages and adversely affected sperm motility, viability and consequently lower hatching rates. The objective of this study is to determine whether propolis has an effect on cryopreservation and fertilization ability and to investigate the potential protective effect of propolis on spermatozoa of Cyprinus carpio. Many studies have been done in cryopreservation offish spermatozoa, but none of them contain propolis in extender composition. The extenders were prepared by using modified Kurokura Solution to which 10% Me2SO added with different levels of propolis (0.2, 0.4, 0.6, 0.8 and 1mgml−1) and 10% egg yolk (as a control without propolis). The pooled semen samples diluted at the ratio of 1:9 by the extenders were subjected to cryopreservation. The percentage and duration of motility and fertilization tests of cryopreserved sperm samples have been done immediately after thawing and compared with control and fresh semen. The extenders containing propolis exhibited higher percentage motility and motility duration than control group (P <0.05). Especially the group IV (0.8mgml−1 propolis) and the group V (1mgml−1 propolis) showed significant positive effects on both post thaw motility and hatching ability. The propolis maintained the integrity of the spermatozoa during the cryopreservation process. Evaluating with its contents, it has been shown that propolis is an appropriate cryoprotective agent in fish semen. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.cryobiol.2014.01.003 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 107 Subjects: – SubjectFull: Propolis Type: general – SubjectFull: Cryopreservation of organs, tissues, etc. Type: general – SubjectFull: Carp Type: general – SubjectFull: Artificial insemination Type: general – SubjectFull: Sperm motility Type: general – SubjectFull: Comparative studies Type: general Titles: – TitleFull: Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Öğretmen, Fatih – PersonEntity: Name: NameFull: İnanan, Burak E. – PersonEntity: Name: NameFull: Öztürk, Mehmet IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 00112240 Numbering: – Type: volume Value: 68 – Type: issue Value: 1 Titles: – TitleFull: Cryobiology Type: main |
| ResultId | 1 |
