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Performance of Two Fluorescence-Based Real-Time Bioaerosol Detectors: BioScout vs. UVAPS.

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Title: Performance of Two Fluorescence-Based Real-Time Bioaerosol Detectors: BioScout vs. UVAPS.
Authors: Saari, S.1 sampo.saari@tut.fi, Reponen, T.2,3, Keskinen, J.1
Source: Aerosol Science & Technology. Apr2014, Vol. 48 Issue 4, p371-378. 8p.
Subjects: Fluorescence, Microbiological aerosols, Biosensors, Semiconductor lasers, Aerodynamics, Particle size determination, Fungal spores
Abstract: A 405 nm diode laser-based on-line bioaerosol detector, BioScout, was tested and compared with the Ultraviolet Aerodynamic Particle Sizer (UVAPS). Both instruments are based on laser-induced fluorescence of particles. Only a fraction of microbial particles produce enough fluorescence light to be detected by the instruments. This fluorescent particle fraction (FPF) is aerosol and instrument specific. The FPF values for common bacterial and fungal spores and biochemical particles were experimentally determined for both instruments. The BioScout exhibited higher FPF values for all the test aerosols except coenzyme NADH. The difference was higher for smaller particles. The FPF values of fungal spores and bacteria varied between 0.34 to 0.77 and 0.13 to 0.54 for the BioScout and the UVAPS, respectively. The results indicate that the 405 nm diode laser is a useful excitation source for fluorescence-based real-time detection of microbial aerosols. The FPF results of this study can be utilized to estimate the actual concentrations of bacterial and fungal spores in fluorescence-based ambient measurements. Copyright 2014 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]
Copyright of Aerosol Science & Technology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Performance of Two Fluorescence-Based Real-Time Bioaerosol Detectors: BioScout vs. UVAPS.
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  Data: <searchLink fieldCode="JN" term="%22Aerosol+Science+%26+Technology%22">Aerosol Science & Technology</searchLink>. Apr2014, Vol. 48 Issue 4, p371-378. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Fluorescence%22">Fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiological+aerosols%22">Microbiological aerosols</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Semiconductor+lasers%22">Semiconductor lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Aerodynamics%22">Aerodynamics</searchLink><br /><searchLink fieldCode="DE" term="%22Particle+size+determination%22">Particle size determination</searchLink><br /><searchLink fieldCode="DE" term="%22Fungal+spores%22">Fungal spores</searchLink>
– Name: Abstract
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  Data: A 405 nm diode laser-based on-line bioaerosol detector, BioScout, was tested and compared with the Ultraviolet Aerodynamic Particle Sizer (UVAPS). Both instruments are based on laser-induced fluorescence of particles. Only a fraction of microbial particles produce enough fluorescence light to be detected by the instruments. This fluorescent particle fraction (FPF) is aerosol and instrument specific. The FPF values for common bacterial and fungal spores and biochemical particles were experimentally determined for both instruments. The BioScout exhibited higher FPF values for all the test aerosols except coenzyme NADH. The difference was higher for smaller particles. The FPF values of fungal spores and bacteria varied between 0.34 to 0.77 and 0.13 to 0.54 for the BioScout and the UVAPS, respectively. The results indicate that the 405 nm diode laser is a useful excitation source for fluorescence-based real-time detection of microbial aerosols. The FPF results of this study can be utilized to estimate the actual concentrations of bacterial and fungal spores in fluorescence-based ambient measurements. Copyright 2014 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Aerosol Science & Technology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1080/02786826.2013.877579
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      – Code: eng
        Text: English
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      – SubjectFull: Fluorescence
        Type: general
      – SubjectFull: Microbiological aerosols
        Type: general
      – SubjectFull: Biosensors
        Type: general
      – SubjectFull: Semiconductor lasers
        Type: general
      – SubjectFull: Aerodynamics
        Type: general
      – SubjectFull: Particle size determination
        Type: general
      – SubjectFull: Fungal spores
        Type: general
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      – TitleFull: Performance of Two Fluorescence-Based Real-Time Bioaerosol Detectors: BioScout vs. UVAPS.
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            NameFull: Saari, S.
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            NameFull: Reponen, T.
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            NameFull: Keskinen, J.
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              Text: Apr2014
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              Y: 2014
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