Quorum vs. diffusion sensing: a quantitative analysis of the relevance of absorbing or reflecting boundaries.
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| Title: | Quorum vs. diffusion sensing: a quantitative analysis of the relevance of absorbing or reflecting boundaries. |
|---|---|
| Authors: | Trovato, Antonio1,2, Seno, Flavio1,2, Zanardo, Marina3, Alberghini, Sara3, Tondello, Alessandra3, Squartini, Andrea3 |
| Source: | FEMS Microbiology Letters. Mar2014, Vol. 352 Issue 2, p198-203. 6p. |
| Subjects: | Bacterial cell interaction, Quorum sensing, Microbial genetics, Absorbing boundary conditions (FDTD method), Cellular control mechanisms |
| Abstract: | The consequences of the boundary conditions (signal reflecting vs. signal adsorbing) on bacterial intercellular communication were addressed by a combined physics and microbiology approach. A predictive biophysical model was devised that considered system size, diffusion from given points, signal molecule decay and boundary properties. The theoretical predictions were tested with two experimental agarose-gel-based set-ups for reflecting or absorbing boundaries. N-acyl homoserine lactone (AHL) concentration profiles were measured using the Agrobacterium tumefaciens NTL4 bioassay and found to agree with model predictions. The half-life of AHL was estimated to be 7 days. The absorbing vs. reflecting nature of the boundaries drastically changed AHL concentration profiles. The effect of a single nonreflecting boundary side was equivalent to a 100-fold lower cell concentration. Results suggest that the kinetics of signal accumulation vs. signal removal and their threshold-mediated phenotypic consequences are directly linked to the properties of biofilm boundaries, stressing the relevance of the diffusion sensing component in bacterial communication. [ABSTRACT FROM AUTHOR] |
| Copyright of FEMS Microbiology Letters is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 95068512 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/1574-6968.12394 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 198 Subjects: – SubjectFull: Bacterial cell interaction Type: general – SubjectFull: Quorum sensing Type: general – SubjectFull: Microbial genetics Type: general – SubjectFull: Absorbing boundary conditions (FDTD method) Type: general – SubjectFull: Cellular control mechanisms Type: general Titles: – TitleFull: Quorum vs. diffusion sensing: a quantitative analysis of the relevance of absorbing or reflecting boundaries. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Trovato, Antonio – PersonEntity: Name: NameFull: Seno, Flavio – PersonEntity: Name: NameFull: Zanardo, Marina – PersonEntity: Name: NameFull: Alberghini, Sara – PersonEntity: Name: NameFull: Tondello, Alessandra – PersonEntity: Name: NameFull: Squartini, Andrea IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 03 Text: Mar2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 03781097 Numbering: – Type: volume Value: 352 – Type: issue Value: 2 Titles: – TitleFull: FEMS Microbiology Letters Type: main |
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