Effects of Varying Growth Conditions on Stable Carbon Isotope Fractionation of Trichloroethene (TCE) by tceA-containing Dehalococcoides mccartyi strains.
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| Title: | Effects of Varying Growth Conditions on Stable Carbon Isotope Fractionation of Trichloroethene (TCE) by tceA-containing Dehalococcoides mccartyi strains. |
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| Authors: | Harding, Katie C.1, Lee, Patrick K. H.1,2, Bill, Markus3, Buscheck, Timothy E.4, Conrad, Mark E.3, Alvarez-Cohen, Lisa1,3 alvarez@ce.berkeley.edu |
| Source: | Environmental Science & Technology. 11/5/2013, Vol. 47 Issue 21, p12342-12350. 9p. |
| Subjects: | Bioremediation, Trichloroethylene, Isotopic fractionation, Dechlorination (Chemistry), Dehalococcoides |
| Abstract: | To quantify in situ bioremediation using compound specific isotope analysis (CSIA), isotope fractionation data obtained from the field is interpreted according to laboratory-derived enrichment factors. Although previous studies that have quantified dynamic isotopic shifts during the reductive dechlorination of trichloroethene (TCE) indicate that fractionation factors can be highly variable from culture-to-culture and site-to-site, the effects of growth condition on the isotope fractionation during reductive dechlorination have not been previously examined. Here, carbon isotope fractionation by Dehalococcoides mccartyi 195 (Dhc195) maintained under a variety of growth conditions was examined. Enrichment factors quantified when Dhc195 was subjected to four suboptimal growth conditions, including decreased temperature (−13.3 ± 0.9‰), trace vitamin B12 availability (−12.7 ± 1.0‰), limited fixed nitrogen (−14.4 ± 0.8‰), and elevated vinyl chloride exposure (−12.5 ± 0.4‰), indicate that the fractionation is similar across a range of tested conditions. The TCE enrichment factors for two syntrophic cocultures, Dhc195 with Desulfovibrio vulgaris Hildenborough (−13.0 ± 2.0‰) and Dhc195 with Syntrophomonas wolfei (−10.4 ± 1.2‰ and −13.3 ± 1.0‰), were also similar to a control experiment. In order to test the stability of enrichment factors in microbial communities, the isotope fractionation was quantified for Dhc-containing groundwater communities before and after two-year enrichment periods under different growth conditions. Although these enrichment factors (−8.9 ± 0.4‰, −6.8 ± 0.8‰, −8.7 ± 1.3‰, −9.4 ± 0.7‰, and −7.2 ± 0.3‰) were predominantly outside the range of values quantified for the isolate and cocultures, all tested enrichment conditions within the communities produced nearly similar fractionations. Enrichment factors were not significantly affected by changes in any of the tested growth conditions for the pure cultures, cocultures or the mixed communities, indicating that despite a variety of temperature, nutrient, and cofactor-limiting conditions, stable carbon isotope fractionations remain consistent for given Dehalococcoides cultures. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Science & Technology is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 95301135 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effects of Varying Growth Conditions on Stable Carbon Isotope Fractionation of Trichloroethene (TCE) by tceA-containing Dehalococcoides mccartyi strains. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Harding%2C+Katie+C%2E%22">Harding, Katie C.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lee%2C+Patrick+K%2E+H%2E%22">Lee, Patrick K. H.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bill%2C+Markus%22">Bill, Markus</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Buscheck%2C+Timothy+E%2E%22">Buscheck, Timothy E.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Conrad%2C+Mark+E%2E%22">Conrad, Mark E.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Alvarez-Cohen%2C+Lisa%22">Alvarez-Cohen, Lisa</searchLink><relatesTo>1,3</relatesTo><i> alvarez@ce.berkeley.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Science+%26+Technology%22">Environmental Science & Technology</searchLink>. 11/5/2013, Vol. 47 Issue 21, p12342-12350. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Bioremediation%22">Bioremediation</searchLink><br /><searchLink fieldCode="DE" term="%22Trichloroethylene%22">Trichloroethylene</searchLink><br /><searchLink fieldCode="DE" term="%22Isotopic+fractionation%22">Isotopic fractionation</searchLink><br /><searchLink fieldCode="DE" term="%22Dechlorination+%28Chemistry%29%22">Dechlorination (Chemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Dehalococcoides%22">Dehalococcoides</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: To quantify in situ bioremediation using compound specific isotope analysis (CSIA), isotope fractionation data obtained from the field is interpreted according to laboratory-derived enrichment factors. Although previous studies that have quantified dynamic isotopic shifts during the reductive dechlorination of trichloroethene (TCE) indicate that fractionation factors can be highly variable from culture-to-culture and site-to-site, the effects of growth condition on the isotope fractionation during reductive dechlorination have not been previously examined. Here, carbon isotope fractionation by Dehalococcoides mccartyi 195 (Dhc195) maintained under a variety of growth conditions was examined. Enrichment factors quantified when Dhc195 was subjected to four suboptimal growth conditions, including decreased temperature (−13.3 ± 0.9‰), trace vitamin B12 availability (−12.7 ± 1.0‰), limited fixed nitrogen (−14.4 ± 0.8‰), and elevated vinyl chloride exposure (−12.5 ± 0.4‰), indicate that the fractionation is similar across a range of tested conditions. The TCE enrichment factors for two syntrophic cocultures, Dhc195 with Desulfovibrio vulgaris Hildenborough (−13.0 ± 2.0‰) and Dhc195 with Syntrophomonas wolfei (−10.4 ± 1.2‰ and −13.3 ± 1.0‰), were also similar to a control experiment. In order to test the stability of enrichment factors in microbial communities, the isotope fractionation was quantified for Dhc-containing groundwater communities before and after two-year enrichment periods under different growth conditions. Although these enrichment factors (−8.9 ± 0.4‰, −6.8 ± 0.8‰, −8.7 ± 1.3‰, −9.4 ± 0.7‰, and −7.2 ± 0.3‰) were predominantly outside the range of values quantified for the isolate and cocultures, all tested enrichment conditions within the communities produced nearly similar fractionations. Enrichment factors were not significantly affected by changes in any of the tested growth conditions for the pure cultures, cocultures or the mixed communities, indicating that despite a variety of temperature, nutrient, and cofactor-limiting conditions, stable carbon isotope fractionations remain consistent for given Dehalococcoides cultures. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Science & Technology is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/es402617q Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 12342 Subjects: – SubjectFull: Bioremediation Type: general – SubjectFull: Trichloroethylene Type: general – SubjectFull: Isotopic fractionation Type: general – SubjectFull: Dechlorination (Chemistry) Type: general – SubjectFull: Dehalococcoides Type: general Titles: – TitleFull: Effects of Varying Growth Conditions on Stable Carbon Isotope Fractionation of Trichloroethene (TCE) by tceA-containing Dehalococcoides mccartyi strains. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Harding, Katie C. – PersonEntity: Name: NameFull: Lee, Patrick K. H. – PersonEntity: Name: NameFull: Bill, Markus – PersonEntity: Name: NameFull: Buscheck, Timothy E. – PersonEntity: Name: NameFull: Conrad, Mark E. – PersonEntity: Name: NameFull: Alvarez-Cohen, Lisa IsPartOfRelationships: – BibEntity: Dates: – D: 05 M: 11 Text: 11/5/2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 0013936X Numbering: – Type: volume Value: 47 – Type: issue Value: 21 Titles: – TitleFull: Environmental Science & Technology Type: main |
| ResultId | 1 |
