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Flow cytometric assessment of microbial abundance in the near-field area of seawater reverse osmosis concentrate discharge.

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Title: Flow cytometric assessment of microbial abundance in the near-field area of seawater reverse osmosis concentrate discharge.
Authors: van der Merwe, R.1 riaan.vandermerwe@kaust.edu.sa, Hammes, F.2, Lattemann, S.1, Amy, G.1
Source: Desalination. Jun2014, Vol. 343, p208-216. 9p.
Subjects: Reverse osmosis in saline water conversion, Flow cytometry, Water quality, Photosynthesis, Water temperature stratification, Aquatic microbiology, Membrane separation
Abstract: Abstract: The discharge of concentrate and other process waters from seawater reverse osmosis (SWRO) plant operations into the marine environment may adversely affect water quality in the near-field area surrounding the outfall. The main concerns are the increase in salt concentration in receiving waters, which results in a density increase and potential water stratification near the outfall, and possible increases in turbidity, e.g., due to the discharge of filter backwash waters. Changes in ambient water quality may affect microbial abundance in the area, for example by hindering the photosynthesis process or disrupting biogenesis. It is widely accepted that marine biodiversity is lower in more extreme conditions, such as high salinity environments. As aquatic microbial communities respond very rapidly to changes in their environment, they can be used as indicators for monitoring ambient water quality. The objective of this study was to assess possible changes in microbial abundance as a result of concentrate discharge into the near-field area (<25m) surrounding the outfall of the King Abdullah University of Science and Technology (KAUST) SWRO plant. Flow cytometric (FCM) analysis was conducted in order to rapidly determine microbial abundance on a single-cell level in 107 samples, taken by diving, from the discharge area, the intake area and two control sites. FCM analysis combined the measurement of distinct scatter of cells and particles, autofluorescence of cyanobacteria and algae, and fluorescence after staining of nucleic acids with SYBR® Green for a total bacterial count. The results indicate that changes in microbial abundance in the near-field area of the KAUST SWRO outfall are minor and appear to be the result of a dilution effect rather than a direct impact of the concentrate discharge. [Copyright &y& Elsevier]
Copyright of Desalination is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Data: Flow cytometric assessment of microbial abundance in the near-field area of seawater reverse osmosis concentrate discharge.
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Desalination%22&quot;&gt;Desalination&lt;/searchLink&gt;. Jun2014, Vol. 343, p208-216. 9p.
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  Data: Abstract: The discharge of concentrate and other process waters from seawater reverse osmosis (SWRO) plant operations into the marine environment may adversely affect water quality in the near-field area surrounding the outfall. The main concerns are the increase in salt concentration in receiving waters, which results in a density increase and potential water stratification near the outfall, and possible increases in turbidity, e.g., due to the discharge of filter backwash waters. Changes in ambient water quality may affect microbial abundance in the area, for example by hindering the photosynthesis process or disrupting biogenesis. It is widely accepted that marine biodiversity is lower in more extreme conditions, such as high salinity environments. As aquatic microbial communities respond very rapidly to changes in their environment, they can be used as indicators for monitoring ambient water quality. The objective of this study was to assess possible changes in microbial abundance as a result of concentrate discharge into the near-field area (&lt;25m) surrounding the outfall of the King Abdullah University of Science and Technology (KAUST) SWRO plant. Flow cytometric (FCM) analysis was conducted in order to rapidly determine microbial abundance on a single-cell level in 107 samples, taken by diving, from the discharge area, the intake area and two control sites. FCM analysis combined the measurement of distinct scatter of cells and particles, autofluorescence of cyanobacteria and algae, and fluorescence after staining of nucleic acids with SYBR&#174; Green for a total bacterial count. The results indicate that changes in microbial abundance in the near-field area of the KAUST SWRO outfall are minor and appear to be the result of a dilution effect rather than a direct impact of the concentrate discharge. [Copyright &amp;y&amp; Elsevier]
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  Data: &lt;i&gt;Copyright of Desalination is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.desal.2014.01.017
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      – Code: eng
        Text: English
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        PageCount: 9
        StartPage: 208
    Subjects:
      – SubjectFull: Reverse osmosis in saline water conversion
        Type: general
      – SubjectFull: Flow cytometry
        Type: general
      – SubjectFull: Water quality
        Type: general
      – SubjectFull: Photosynthesis
        Type: general
      – SubjectFull: Water temperature stratification
        Type: general
      – SubjectFull: Aquatic microbiology
        Type: general
      – SubjectFull: Membrane separation
        Type: general
    Titles:
      – TitleFull: Flow cytometric assessment of microbial abundance in the near-field area of seawater reverse osmosis concentrate discharge.
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            NameFull: Hammes, F.
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              Text: Jun2014
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              Y: 2014
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              Value: 343
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