Combining mass spectrometry with database interrogation strategies in proteomics

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Title: Combining mass spectrometry with database interrogation strategies in proteomics
Authors: Liska, Adam J.1, Shevchenko, Andrej shevchenko@mpi-cbg.de
Source: Trends in Analytical Chemistry: TRAC. May2003, Vol. 22 Issue 5, p291. 8p.
Subjects: Proteomics, Mass spectrometry
Abstract: Protein identification by mass spectrometry (MS) and sequence-database searching was established in 1993; since then, the proteomics community has witnessed a proliferation of analytical strategies for protein identification. Analytical strategies comprise three components: MS platforms; spectra-database sequence correlation methods; and, sequence databases. Multiple strategies are now applied simultaneously to increase sensitivity, throughput, and reliability of the characterization of proteomes. Now, by assessing the complexity of the interplay of MS, bioinformatics and sequence databases, we can begin to predict future approaches and challenges in the development of proteomics. [Copyright &y& Elsevier]
Copyright of Trends in Analytical Chemistry: TRAC is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Protein identification by mass spectrometry (MS) and sequence-database searching was established in 1993; since then, the proteomics community has witnessed a proliferation of analytical strategies for protein identification. Analytical strategies comprise three components: MS platforms; spectra-database sequence correlation methods; and, sequence databases. Multiple strategies are now applied simultaneously to increase sensitivity, throughput, and reliability of the characterization of proteomes. Now, by assessing the complexity of the interplay of MS, bioinformatics and sequence databases, we can begin to predict future approaches and challenges in the development of proteomics. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Trends in Analytical Chemistry: TRAC is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/S0165-9936(03)00507-7
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