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Structure of the oligogalacturonate-specific KdgM porin.

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Title: Structure of the oligogalacturonate-specific KdgM porin.
Authors: Hutter, C. A. J., Lehner, R., Wirth, Ch., Condemine, G., Peneff, C., Schirmer, T.
Source: Acta Crystallographica: Section D (Wiley-Blackwell). Jun2014, Vol. 70 Issue 6, p1770-1778. 9p.
Subjects: Porins (Proteins), Phytopathogenic bacteria, Erwinia chrysanthemi, Plant cell walls, Oligosaccharides
Abstract: The phytopathogenic Gram-negative bacterium Dickeya dadantii ( Erwinia chrysanthemi) feeds on plant cell walls by secreting pectinases and utilizing the oligogalacturanate products. An outer membrane porin, KdgM, is indispensable for the uptake of these acidic oligosaccharides. Here, the crystal structure of KdgM determined to 1.9 Å resolution is presented. KdgM is folded into a regular 12-stranded antiparallel β-barrel with a circular cross-section defining a transmembrane pore with a minimal radius of 3.1 Å. Most of the loops that would face the cell exterior in vivo are disordered, but nevertheless mediate contact between densely packed membrane-like layers in the crystal. The channel is lined by two tracks of arginine residues facing each other across the pore, a feature that is conserved within the KdgM family and is likely to facilitate the diffusion of acidic oligosaccharides. [ABSTRACT FROM AUTHOR]
Copyright of Acta Crystallographica: Section D (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: <searchLink fieldCode="DE" term="%22Porins+%28Proteins%29%22">Porins (Proteins)</searchLink><br /><searchLink fieldCode="DE" term="%22Phytopathogenic+bacteria%22">Phytopathogenic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Erwinia+chrysanthemi%22">Erwinia chrysanthemi</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+cell+walls%22">Plant cell walls</searchLink><br /><searchLink fieldCode="DE" term="%22Oligosaccharides%22">Oligosaccharides</searchLink>
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  Data: The phytopathogenic Gram-negative bacterium Dickeya dadantii ( Erwinia chrysanthemi) feeds on plant cell walls by secreting pectinases and utilizing the oligogalacturanate products. An outer membrane porin, KdgM, is indispensable for the uptake of these acidic oligosaccharides. Here, the crystal structure of KdgM determined to 1.9 Å resolution is presented. KdgM is folded into a regular 12-stranded antiparallel β-barrel with a circular cross-section defining a transmembrane pore with a minimal radius of 3.1 Å. Most of the loops that would face the cell exterior in vivo are disordered, but nevertheless mediate contact between densely packed membrane-like layers in the crystal. The channel is lined by two tracks of arginine residues facing each other across the pore, a feature that is conserved within the KdgM family and is likely to facilitate the diffusion of acidic oligosaccharides. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Acta Crystallographica: Section D (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1107/S1399004714007147
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      – Code: eng
        Text: English
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        PageCount: 9
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      – SubjectFull: Porins (Proteins)
        Type: general
      – SubjectFull: Phytopathogenic bacteria
        Type: general
      – SubjectFull: Erwinia chrysanthemi
        Type: general
      – SubjectFull: Plant cell walls
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              Text: Jun2014
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