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Ferrocene Entrapped In Polypyrrole Film and PAMAM Dendrimers as Matrix for Mediated Glucose/O2 Biofuel Cell.

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Title: Ferrocene Entrapped In Polypyrrole Film and PAMAM Dendrimers as Matrix for Mediated Glucose/O2 Biofuel Cell.
Authors: Crepaldi, L. B.1, Neto, S. Aquino1, Cardoso, F. P.1, Ciancaglini, P.1, De Andrade, A. R.1 adalgisa.andrade2@gmail.com
Source: Electrochimica Acta. Aug2014, Vol. 136, p52-58. 7p.
Subjects: Ferrocene, Polypyrrole, Thin films, Polyamidoamine dendrimers, Glucose oxidase, Biomass energy, Cyclic voltammetry
Abstract: We fabricated bioanodes based on PAMAM (polyamidoamine) dendrimers to immobilize glucose oxidase (GOx), using ferrocenium hexafluorophosphate (Fc) entrapped in electropolymerized polypyrrole films (Fc-polypyrrole) as mediator. We conducted a comparative study of the GOx kinetic parameters as a function of the enzyme environment; i.e., in solution and immobilized onto carbon platforms. Kinetic studies revealed that the Michaelis-Menten constants (KM) of the anchored and soluble GOx were quite similar, KM=21.2 (± 0.6) mmol L−1 in solution, and 21.5 (± 0.8) mmol L−1 when immobilized, demonstrating that enzyme immobilization did not affect the enzyme-substrate affinity. However, the maximum reaction rate (Vmax) obtained for the immobilized GOx (Vmax=0.72 (± 0.03) μmol min−1mg−1) was lower than the reaction rate achieved for the free enzyme in solution (Vmax=64 (± 2) μmol min−1mg−1), due to diffusional effects. Cyclic voltammetry assays confirmed the incorporation of entrapped Fc species into the polypyrrole net films. The performance of the prepared bioelectrodes in a glucose/O2 semi-cell revealed a maximum power density around 130 (± 15) μW cm−2 at pH 5.0, in 100mmolL−1 glucose. Considering all the electrochemical data and biofuel cell tests, we can infer that the bioanode based on Fc and polypyrrole presented here, is easy to prepare, and the electrocatalyst film efficiently transfers electrons between GOx and the electrode surface. Therefore, it has potential application in glucose/O2 biofuel cells. [ABSTRACT FROM AUTHOR]
Copyright of Electrochimica Acta is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 97128886
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PubTypeId: academicJournal
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  Label: Title
  Group: Ti
  Data: Ferrocene Entrapped In Polypyrrole Film and PAMAM Dendrimers as Matrix for Mediated Glucose/O2 Biofuel Cell.
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  Data: <searchLink fieldCode="AR" term="%22Crepaldi%2C+L%2E+B%2E%22">Crepaldi, L. B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Neto%2C+S%2E+Aquino%22">Neto, S. Aquino</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cardoso%2C+F%2E+P%2E%22">Cardoso, F. P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ciancaglini%2C+P%2E%22">Ciancaglini, P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22De+Andrade%2C+A%2E+R%2E%22">De Andrade, A. R.</searchLink><relatesTo>1</relatesTo><i> adalgisa.andrade2@gmail.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Electrochimica+Acta%22">Electrochimica Acta</searchLink>. Aug2014, Vol. 136, p52-58. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Ferrocene%22">Ferrocene</searchLink><br /><searchLink fieldCode="DE" term="%22Polypyrrole%22">Polypyrrole</searchLink><br /><searchLink fieldCode="DE" term="%22Thin+films%22">Thin films</searchLink><br /><searchLink fieldCode="DE" term="%22Polyamidoamine+dendrimers%22">Polyamidoamine dendrimers</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose+oxidase%22">Glucose oxidase</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass+energy%22">Biomass energy</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclic+voltammetry%22">Cyclic voltammetry</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: We fabricated bioanodes based on PAMAM (polyamidoamine) dendrimers to immobilize glucose oxidase (GOx), using ferrocenium hexafluorophosphate (Fc) entrapped in electropolymerized polypyrrole films (Fc-polypyrrole) as mediator. We conducted a comparative study of the GOx kinetic parameters as a function of the enzyme environment; i.e., in solution and immobilized onto carbon platforms. Kinetic studies revealed that the Michaelis-Menten constants (KM) of the anchored and soluble GOx were quite similar, KM=21.2 (± 0.6) mmol L−1 in solution, and 21.5 (± 0.8) mmol L−1 when immobilized, demonstrating that enzyme immobilization did not affect the enzyme-substrate affinity. However, the maximum reaction rate (Vmax) obtained for the immobilized GOx (Vmax=0.72 (± 0.03) μmol min−1mg−1) was lower than the reaction rate achieved for the free enzyme in solution (Vmax=64 (± 2) μmol min−1mg−1), due to diffusional effects. Cyclic voltammetry assays confirmed the incorporation of entrapped Fc species into the polypyrrole net films. The performance of the prepared bioelectrodes in a glucose/O2 semi-cell revealed a maximum power density around 130 (± 15) μW cm−2 at pH 5.0, in 100mmolL−1 glucose. Considering all the electrochemical data and biofuel cell tests, we can infer that the bioanode based on Fc and polypyrrole presented here, is easy to prepare, and the electrocatalyst film efficiently transfers electrons between GOx and the electrode surface. Therefore, it has potential application in glucose/O2 biofuel cells. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Electrochimica Acta is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.electacta.2014.05.049
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 7
        StartPage: 52
    Subjects:
      – SubjectFull: Ferrocene
        Type: general
      – SubjectFull: Polypyrrole
        Type: general
      – SubjectFull: Thin films
        Type: general
      – SubjectFull: Polyamidoamine dendrimers
        Type: general
      – SubjectFull: Glucose oxidase
        Type: general
      – SubjectFull: Biomass energy
        Type: general
      – SubjectFull: Cyclic voltammetry
        Type: general
    Titles:
      – TitleFull: Ferrocene Entrapped In Polypyrrole Film and PAMAM Dendrimers as Matrix for Mediated Glucose/O2 Biofuel Cell.
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            NameFull: Crepaldi, L. B.
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            NameFull: Neto, S. Aquino
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            NameFull: Cardoso, F. P.
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            NameFull: De Andrade, A. R.
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              M: 08
              Text: Aug2014
              Type: published
              Y: 2014
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              Value: 136
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