Impact of fruit processing methods on DNA extraction from transgenic frozen banana products.
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| Title: | Impact of fruit processing methods on DNA extraction from transgenic frozen banana products. |
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| Authors: | Abdolmaleki, Farzaneh1 fa.abdolmaleki@gmail.com, Assadi, Mahnaz2, Ezzatpanah, Hamid1, Honarvar, Masoud1 |
| Source: | European Food Research & Technology. Sep2014, Vol. 239 Issue 3, p509-517. 9p. |
| Subjects: | Fruit processing plants, Bananas, Nucleic acid isolation methods, Transgenic plants, Frozen fruit, Food chemistry, Food quality |
| Abstract: | Isolation of high yield and quality of genomic DNA is paramount for ensuring confidence in molecular analyzing food. This study evaluated five different DNA extraction procedures based on laboratory protocols and commercial kits for their efficiency and also assessed the impact of processing procedures on the DNA degradation in various frozen banana products such as frozen-sliced banana, frozen banana puree, frozen plantain banana, and freeze-dried banana powder. The purity and concentration of DNA obtained from the samples were evaluated by spectrophotometric and gel electrophoresis analysis. Of the various extraction procedures, we applied modified protocols that allow for effective extraction of DNA from banana. This protocol derived from traditional cetyltrimethylammonium bromide method with modifications that allowed removing unwanted polyphenols and polysaccharides. The results confirm that a profound impact on DNA degradation was seen during the processing of banana products, DNA could still be reliably quantified by real-time PCR. Furthermore, frying resulted in further degradations and drying resulted in the most severe changes. It was noted that in different frozen banana products, nopaline synthase fragment is most stable followed by cauliflower mosaic virus 35S and NPTII gene. [ABSTRACT FROM AUTHOR] |
| Copyright of European Food Research & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 97460496 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Impact of fruit processing methods on DNA extraction from transgenic frozen banana products. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Abdolmaleki%2C+Farzaneh%22">Abdolmaleki, Farzaneh</searchLink><relatesTo>1</relatesTo><i> fa.abdolmaleki@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Assadi%2C+Mahnaz%22">Assadi, Mahnaz</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ezzatpanah%2C+Hamid%22">Ezzatpanah, Hamid</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Honarvar%2C+Masoud%22">Honarvar, Masoud</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Food+Research+%26+Technology%22">European Food Research & Technology</searchLink>. Sep2014, Vol. 239 Issue 3, p509-517. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Fruit+processing+plants%22">Fruit processing plants</searchLink><br /><searchLink fieldCode="DE" term="%22Bananas%22">Bananas</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acid+isolation+methods%22">Nucleic acid isolation methods</searchLink><br /><searchLink fieldCode="DE" term="%22Transgenic+plants%22">Transgenic plants</searchLink><br /><searchLink fieldCode="DE" term="%22Frozen+fruit%22">Frozen fruit</searchLink><br /><searchLink fieldCode="DE" term="%22Food+chemistry%22">Food chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Food+quality%22">Food quality</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Isolation of high yield and quality of genomic DNA is paramount for ensuring confidence in molecular analyzing food. This study evaluated five different DNA extraction procedures based on laboratory protocols and commercial kits for their efficiency and also assessed the impact of processing procedures on the DNA degradation in various frozen banana products such as frozen-sliced banana, frozen banana puree, frozen plantain banana, and freeze-dried banana powder. The purity and concentration of DNA obtained from the samples were evaluated by spectrophotometric and gel electrophoresis analysis. Of the various extraction procedures, we applied modified protocols that allow for effective extraction of DNA from banana. This protocol derived from traditional cetyltrimethylammonium bromide method with modifications that allowed removing unwanted polyphenols and polysaccharides. The results confirm that a profound impact on DNA degradation was seen during the processing of banana products, DNA could still be reliably quantified by real-time PCR. Furthermore, frying resulted in further degradations and drying resulted in the most severe changes. It was noted that in different frozen banana products, nopaline synthase fragment is most stable followed by cauliflower mosaic virus 35S and NPTII gene. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Food Research & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00217-014-2246-4 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 509 Subjects: – SubjectFull: Fruit processing plants Type: general – SubjectFull: Bananas Type: general – SubjectFull: Nucleic acid isolation methods Type: general – SubjectFull: Transgenic plants Type: general – SubjectFull: Frozen fruit Type: general – SubjectFull: Food chemistry Type: general – SubjectFull: Food quality Type: general Titles: – TitleFull: Impact of fruit processing methods on DNA extraction from transgenic frozen banana products. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Abdolmaleki, Farzaneh – PersonEntity: Name: NameFull: Assadi, Mahnaz – PersonEntity: Name: NameFull: Ezzatpanah, Hamid – PersonEntity: Name: NameFull: Honarvar, Masoud IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 14382377 Numbering: – Type: volume Value: 239 – Type: issue Value: 3 Titles: – TitleFull: European Food Research & Technology Type: main |
| ResultId | 1 |
