Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy.
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| Title: | Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy. |
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| Authors: | Spring, Bryan Q.1, Palanisami, Akilan1, Hasan, Tayyaba1,2,3 thasan@mgh.harvard.edu |
| Source: | Journal of Biomedical Optics. Jun2014, Vol. 19 Issue 6, p1-5. 5p. |
| Subjects: | Micrometastasis, Leukocytes, Accuracy, Tissue wounds, Biopsy |
| Abstract: | Molecular-targeted probes are emerging with applications for optical biopsy of cancer. An underex-plored potential clinical use of these probes is to monitor residual cancer micrometastases that escape cyto-reductive surgery and chemotherapy. Here, we show that leukocytes, or white blood cells, residing in nontumor tissues--as well as those infiltrating micrometastatic lesions--uptake cancer cell-targeted, activatable immuno-conjugates nonspecifically, which limits the accuracy and resolution of micrometastasis recognition using these probes. Receiver operating characteristic analysis of freshly excised tissues from a mouse model of peritoneal carcinomatosis suggests that dual-color imaging, adding an immunostain for leukocytes, offers promise for enabling accurate recognition of single cancer cells. Our results indicate that leukocyte identification improves micrometastasis recognition sensitivity and specificity from 92 to 93%--for multicellular metastases >20 to 30 μm in size--to 98 to 99.9% for resolving metastases as small as a single cell. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 97480768 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Spring%2C+Bryan+Q%2E%22">Spring, Bryan Q.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Palanisami%2C+Akilan%22">Palanisami, Akilan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hasan%2C+Tayyaba%22">Hasan, Tayyaba</searchLink><relatesTo>1,2,3</relatesTo><i> thasan@mgh.harvard.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Optics%22">Journal of Biomedical Optics</searchLink>. Jun2014, Vol. 19 Issue 6, p1-5. 5p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Micrometastasis%22">Micrometastasis</searchLink><br /><searchLink fieldCode="DE" term="%22Leukocytes%22">Leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Accuracy%22">Accuracy</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+wounds%22">Tissue wounds</searchLink><br /><searchLink fieldCode="DE" term="%22Biopsy%22">Biopsy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Molecular-targeted probes are emerging with applications for optical biopsy of cancer. An underex-plored potential clinical use of these probes is to monitor residual cancer micrometastases that escape cyto-reductive surgery and chemotherapy. Here, we show that leukocytes, or white blood cells, residing in nontumor tissues--as well as those infiltrating micrometastatic lesions--uptake cancer cell-targeted, activatable immuno-conjugates nonspecifically, which limits the accuracy and resolution of micrometastasis recognition using these probes. Receiver operating characteristic analysis of freshly excised tissues from a mouse model of peritoneal carcinomatosis suggests that dual-color imaging, adding an immunostain for leukocytes, offers promise for enabling accurate recognition of single cancer cells. Our results indicate that leukocyte identification improves micrometastasis recognition sensitivity and specificity from 92 to 93%--for multicellular metastases >20 to 30 μm in size--to 98 to 99.9% for resolving metastases as small as a single cell. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1117/1.JBO.19.6.066006 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 1 Subjects: – SubjectFull: Micrometastasis Type: general – SubjectFull: Leukocytes Type: general – SubjectFull: Accuracy Type: general – SubjectFull: Tissue wounds Type: general – SubjectFull: Biopsy Type: general Titles: – TitleFull: Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Spring, Bryan Q. – PersonEntity: Name: NameFull: Palanisami, Akilan – PersonEntity: Name: NameFull: Hasan, Tayyaba IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 10833668 Numbering: – Type: volume Value: 19 – Type: issue Value: 6 Titles: – TitleFull: Journal of Biomedical Optics Type: main |
| ResultId | 1 |
