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Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor.

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Title: Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor.
Authors: Costa, Maurilia P.1, Andrade, Cesar A.S.2, Montenegro, Rosana A.3, Melo, Fabio L.3, Oliveira, Maria D.L.1,2 m_danielly@yahoo.com.br
Source: Journal of Colloid & Interface Science. Nov2014, Vol. 433, p141-148. 8p.
Subjects: Molecular self-assembly, Monomolecular films, Chemical derivatives, Benzoic acid, Magnetite, Electrochemical analysis, Mycobacterium tuberculosis, DNA probes
Abstract: In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe 3 O 4 Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe 3 O 4 Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe–genome interaction was evaluated using a [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe 3 O 4 Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6 ng μL −1 ). [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe 3 O 4 Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe 3 O 4 Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe–genome interaction was evaluated using a [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe 3 O 4 Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6 ng μL −1 ). [ABSTRACT FROM AUTHOR]
ISSN:00219797
DOI:10.1016/j.jcis.2014.07.014