Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor.
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| Title: | Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor. |
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| Authors: | Costa, Maurilia P.1, Andrade, Cesar A.S.2, Montenegro, Rosana A.3, Melo, Fabio L.3, Oliveira, Maria D.L.1,2 m_danielly@yahoo.com.br |
| Source: | Journal of Colloid & Interface Science. Nov2014, Vol. 433, p141-148. 8p. |
| Subjects: | Molecular self-assembly, Monomolecular films, Chemical derivatives, Benzoic acid, Magnetite, Electrochemical analysis, Mycobacterium tuberculosis, DNA probes |
| Abstract: | In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe 3 O 4 Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe 3 O 4 Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe–genome interaction was evaluated using a [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe 3 O 4 Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6 ng μL −1 ). [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Colloid & Interface Science is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 98142114 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Costa%2C+Maurilia+P%2E%22">Costa, Maurilia P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Andrade%2C+Cesar+A%2ES%2E%22">Andrade, Cesar A.S.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Montenegro%2C+Rosana+A%2E%22">Montenegro, Rosana A.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Melo%2C+Fabio+L%2E%22">Melo, Fabio L.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Oliveira%2C+Maria+D%2EL%2E%22">Oliveira, Maria D.L.</searchLink><relatesTo>1,2</relatesTo><i> m_danielly@yahoo.com.br</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Colloid+%26+Interface+Science%22">Journal of Colloid & Interface Science</searchLink>. Nov2014, Vol. 433, p141-148. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Molecular+self-assembly%22">Molecular self-assembly</searchLink><br /><searchLink fieldCode="DE" term="%22Monomolecular+films%22">Monomolecular films</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+derivatives%22">Chemical derivatives</searchLink><br /><searchLink fieldCode="DE" term="%22Benzoic+acid%22">Benzoic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetite%22">Magnetite</searchLink><br /><searchLink fieldCode="DE" term="%22Electrochemical+analysis%22">Electrochemical analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Mycobacterium+tuberculosis%22">Mycobacterium tuberculosis</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+probes%22">DNA probes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe 3 O 4 Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe 3 O 4 Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe–genome interaction was evaluated using a [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe 3 O 4 Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6 ng μL −1 ). [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Colloid & Interface Science is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jcis.2014.07.014 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 141 Subjects: – SubjectFull: Molecular self-assembly Type: general – SubjectFull: Monomolecular films Type: general – SubjectFull: Chemical derivatives Type: general – SubjectFull: Benzoic acid Type: general – SubjectFull: Magnetite Type: general – SubjectFull: Electrochemical analysis Type: general – SubjectFull: Mycobacterium tuberculosis Type: general – SubjectFull: DNA probes Type: general Titles: – TitleFull: Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Costa, Maurilia P. – PersonEntity: Name: NameFull: Andrade, Cesar A.S. – PersonEntity: Name: NameFull: Montenegro, Rosana A. – PersonEntity: Name: NameFull: Melo, Fabio L. – PersonEntity: Name: NameFull: Oliveira, Maria D.L. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 00219797 Numbering: – Type: volume Value: 433 Titles: – TitleFull: Journal of Colloid & Interface Science Type: main |
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